CstF-64 Polyclonal antibody

• Featured Product
• KD/KO Validated

CstF-64 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 26825-1-AP

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64

---

Validation Data Gallery

• 

  WB analysis of COLO 320 using 26825-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26825-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 26825-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 26825-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 26825-1-AP (CstF-64 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 26825-1-AP

  IP Result of anti-CstF-64 (IP:26825-1-AP, 4ug; Detection:26825-1-AP 1:300) with HeLa cells lysate 2900ug.

• 

  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26825-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26825-1-AP (CstF-64 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 26825-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 26825-1-AP (CstF-64 antibody) at dilution of 1:150 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, mouse brain tissue, mouse testis tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 26825-1-AP is CstF-64 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CstF-64 fusion protein Ag25254
• Full Name: cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
• Calculated molecular weight: 61 kDa
• Observed molecular weight: 64 kDa
• GenBank accession number: BC017712
• Gene symbol: CSTF2
• Gene ID (NCBI): 1478
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Publications

Species	Application	Title
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	IF	Biochem Biophys Res Commun; Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation.; Authors - Rahul Neupane ; KD Validated
mouse	IHC	Front Oncol; CSTF2 Promotes Hepatocarcinogenesis and Hepatocellular Carcinoma Progression via Aerobic Glycolysis; Authors - Zhimin Chen
rat,mouse,human		iScience; Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability; Authors - Ziqian Min