CstF-64 Polyclonal antibody

CstF-64 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26825-1-AP

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26825-1-AP is CstF-64 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CstF-64 fusion protein Ag25254
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Calculated molecular weight 61 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC017712
Gene symbol CSTF2
Gene ID (NCBI) 1478
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Protocols

Product Specific Protocols
WB protocol for CstF-64 antibody 26825-1-APDownload protocol
IHC protocol for CstF-64 antibody 26825-1-APDownload protocol
IF protocol for CstF-64 antibody 26825-1-APDownload protocol
IP protocol for CstF-64 antibody 26825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols