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  • KD/KO Validated

CstF-64 Polyclonal antibody

CstF-64 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 26825-1-AP

Synonyms

CF 1 64 kDa subunit, CstF 64, CSTF 64 kDa subunit, CSTF2, CstF-64



Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, mouse brain tissue, mouse testis tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26825-1-AP is CstF-64 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CstF-64 fusion protein Ag25254
Full Name cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
Calculated Molecular Weight 61 kDa
Observed Molecular Weight 64 kDa
GenBank Accession NumberBC017712
Gene Symbol CSTF2
Gene ID (NCBI) 1478
RRIDAB_2880649
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cleavage stimulation factor 64 kDa subunit is a protein that in humans is encoded by the CSTF2 gene. This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs.

Protocols

Product Specific Protocols
WB protocol for CstF-64 antibody 26825-1-APDownload protocol
IHC protocol for CstF-64 antibody 26825-1-APDownload protocol
IF protocol for CstF-64 antibody 26825-1-APDownload protocol
IP protocol for CstF-64 antibody 26825-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanCoIP

Neuron

Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.

Authors - Geneva R LaForce
humanIF

Biochem Biophys Res Commun

Cleavage stimulating factor 64 depletion mitigates cardiac fibrosis through alternative polyadenylation.

Authors - Rahul Neupane
  • KD Validated
mouseIHC

Front Oncol

CSTF2 Promotes Hepatocarcinogenesis and Hepatocellular Carcinoma Progression via Aerobic Glycolysis

Authors - Zhimin Chen
rat,mouse,human

iScience

Chromodomain helicase DNA-binding domain 2 maintains spermatogonial self-renewal by promoting chromatin accessibility and mRNA stability

Authors - Ziqian Min
humanWB

J Hepatocell Carcinoma

A Novel Gene Pair CSTF2/DPE2A Impacts Prognosis and Cell Cycle of Hepatocellular Carcinoma

Authors - Yangjin Huang