Cubilin Polyclonal antibody

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  WB analysis using 31992-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31992-1-AP (CUBN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

Cubilin Polyclonal Antibody for WB, ELISA

Cat No. 31992-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Intrinsic factor-vitamin B12 receptor, Intrinsic factor-cobalamin receptor, Intestinal intrinsic factor receptor, IFCR, CUBN

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Tested Applications

• Positive WB detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

31992-1-AP targets Cubilin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cubilin fusion protein Ag35808
• Full Name: cubilin (intrinsic factor-cobalamin receptor)
• Calculated Molecular Weight: 398kDa
• Observed Molecular Weight: 450 kDa
• GenBank Accession Number: NM_001081
• Gene Symbol: CUBN
• Gene ID (NCBI): 8029
• RRID: AB_3670168
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: O60494
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. Cubilin is an endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake (PMID: 9572993). Cubilin Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1, which also acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex (PMID: 14576052). There are multiple glycosylation sites on Cubilin.

Publications

Species	Application	Title
human	IHC	Arthritis Res Ther; Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning; Authors - Li Zhang