Tested Applications
Positive WB detected in | mouse kidney tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
Product Information
31992-1-AP targets Cubilin in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Cubilin fusion protein Ag35808 Predict reactive species |
Full Name | cubilin (intrinsic factor-cobalamin receptor) |
Calculated Molecular Weight | 398kDa |
Observed Molecular Weight | 450 kDa |
GenBank Accession Number | NM_001081 |
Gene Symbol | CUBN |
Gene ID (NCBI) | 8029 |
RRID | AB_3670168 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity Purification |
UNIPROT ID | O60494 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cubilin (CUBN) acts as a receptor for intrinsic factor-vitamin B12 complexes. Cubulin is located within the epithelium of intestine and kidney. Mutations in CUBN may play a role in autosomal recessive megaloblastic anemia. Cubilin is an endocytic receptor which plays a role in lipoprotein, vitamin and iron metabolism by facilitating their uptake (PMID: 9572993). Cubilin Acts together with LRP2 to mediate endocytosis of high-density lipoproteins, GC, hemoglobin, ALB, TF and SCGB1A1, which also acts together with AMN to mediate endocytosis of the CBLIF-cobalamin complex (PMID: 14576052). There are multiple glycosylation sites on Cubilin.
Protocols
Product Specific Protocols | |
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WB protocol for Cubilin antibody 31992-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Arthritis Res Ther Investigation of ferroptosis-associated molecular subtypes and immunological characteristics in lupus nephritis based on artificial neural network learning |