Cyclin E1 Recombinant antibody

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  IHC staining of rat testis using 82700-9-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 82700-9-RR

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82700-9-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 82700-9-RR

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 82700-9-RR (Cyclin E1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 82700-9-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  IF Staining of HeLa using 82700-9-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E antibody (82700-9-RR, Clone: 240136H5 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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  FC experiment of HeLa using 82700-9-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  FC experiment of MCF-7 using 82700-9-RR

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Cyclin E Recombinant Antibody (82700-9-RR, Clone:240136H5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 82700-9-RR

  Biolayer interferometry (BLl) kinetic assays of 82700-9 -RR against Human Cyclin E were performed. The affinity constant is below 1 pM.

Cyclin E1 Uni-rAb^TM Recombinant Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 82700-9-RR

Clone No.240136H5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF/ICC, FC (Intra), ELISA

Synonyms

CCNE1, 240136H5, CCNE, Cyclin E, G1/S specific cyclin E1

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Tested Applications

• Positive IHC detected in: rat testis tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells
• Positive FC (Intra) detected in: HeLa cells, MCF-7 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82700-9-RR targets Cyclin E1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Cyclin E1 fusion protein Ag2110
• Full Name: cyclin E1
• Calculated Molecular Weight: 410 aa, 47 kDa
• GenBank Accession Number: BC035498
• Gene Symbol: Cyclin E1
• Gene ID (NCBI): 898
• RRID: AB_3670533
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24864
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin E1 (CCNE1) is a member of the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNE1, an essential cyclin activating Cdk2, regulates the G1-S phase transition of the mammalian cell division cycle. Its timing expression plays a direct role in the initiation of DNA replication , the control of histone biosynthesis, and the centrosome cycle. CCNE1 is associated with disease progression in various malignancies and is associated clinically with poor prognosis. Two bands of Cyclin E1 were expressed in U2OS and MDA-MB-231 cells (PMID:9858585, PMID: 24112607).