Tested Applications
| Positive WB detected in | MDA-MB-231 cells, MDA-MB-453s cells, T-47D cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29306-1-AP targets Cyclin G in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30937 Product name: Recombinant human CCNG1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 202-295 aa of BC007093 Sequence: VLALSIIALEIQAQKCVELTEGIECLQKHSKINGRDLTFWQELVSKCLTEYSSNECSKPNVQKLKWIVSGRTARQLKHSYYRITHLPTIPEMVP Predict reactive species |
| Full Name | cyclin G1 |
| Calculated Molecular Weight | 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC007093 |
| Gene Symbol | Cyclin G |
| Gene ID (NCBI) | 900 |
| RRID | AB_3086111 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51959 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cyclin G is also named as CCNG, CYCG1 and CCNG1, and and belongs to the cyclin family. Cyclin G may play a role in growth regulation. Cyclin G is associated with G2/M phase arrest in response to DNA damage. And it may be an intermediate by which p53 mediates its role as an inhibitor of cellular proliferation, and levels of Cyclin G are increased after induction of p53 by DNA damage (PMID:12015958). Cyclin G is high levels in skeletal muscle, ovary, kidney and colon. Cyclin G is very low levels in normal cells during G1 phase, which increase as cells enter the S phase and stay high throughout the S and G2/M phases. In breast cancer cells, consistent high levels of Cyclin G are found throughout the cell cycle (PMID:11063931).
Publications
What published studies show
The Cyclin G Polyclonal antibody (Cat# 29306-1-AP) has 1 total citation. It appears in the journal Biomolecules (Impact Factor 5.6), published in January 2025. The cited study investigates how Cyclin G1 regulates alveolarization in bronchopulmonary dysplasia models by inhibiting AT2 cell proliferation, placing it in the fields of lung development and pulmonary disease research. The antibody was used in WB and IF applications with mouse samples.
| Species | Application | Title |
|---|---|---|
Biomolecules Cyclin G1 Regulates the Alveolarization in Models of Bronchopulmonary Dysplasia by Inhibiting AT2 Cell Proliferation |
