Cyclophilin B Recombinant monoclonal antibody

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• Cyclophilin B Recombinant monoclonal antibody (86744-1-RR)

Cyclophilin B Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86744-1-RR

Clone No.251476F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PPIB, CYP S1, CYPB, CYP-S1, EC:5.2.1.8

• 

  WB analysis using 86744-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86744-1-RR (Cyclophilin B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 86744-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86744-1-RR (Cyclophilin B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Jurkat cells, Raji cells, mouse liver tissue, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86744-1-RR targets Cyclophilin B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag2161

  Product name: Recombinant human PPIB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC032138

  Sequence: MLRLSERNMKVLLAAALIAGSVFFLLLPGPSAADEKKKGPKVTVKVYFDLRIGDEDVGRVIFGLFGKTVPKTVDNFVALATGEKGFGYKNSKFHRVIKDFMIQGGDFTRGDGTGGKSIYGERFPDENFKLKHYGPGWVSMANAGKDTNGSQFFITTVKTAWLDGKHVVFGKVLEGMEVVRKVESTKTDSRDKPLKDVIIADCGKIEVEKPFAIAKE

• Full Name: peptidylprolyl isomerase B (cyclophilin B)
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 19-24 kDa
• GenBank Accession Number: BC032138
• Gene Symbol: Cyclophilin B
• Gene ID (NCBI): 5479
• RRID: AB_3745097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23284
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclophilin B (CYPB) , also named as PPIB, SCYLP, PPIase B and CYP-S1, belongs to the cyclophilin-type PPIase family and PPIase B subfamily. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIB is secreted in biological fluids such as blood or milk and binds to a specific receptor present on the human lymphoblastic cell line Jurkat and on human peripheral blood lymphocytes. The full-length of PPIB has 216aa (24 kDa), and a 33aa-signal peptide could be removed in the matrue form (about 19 kDa).