Cytokeratin 10 Recombinant monoclonal antibody

• 

  WB analysis using 86324-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86324-1-RR (KRT10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 86324-1-RR

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 86324-1-RR (Cytokeratin 10 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse skin using 86324-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse skin tissue using Cytokeratin 10 antibody (86324-1-RR, Clone: 250893H2 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 10 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. 86324-1-RR

Clone No.250893H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

KRT10, CK 10, CK10, CK-10, Cytokeratin,Keratin

---

Tested Applications

• Positive WB detected in: mouse skin tissue, rat skin tissue
• Positive IHC detected in: human skin cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86324-1-RR targets Cytokeratin 10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 10
• Calculated Molecular Weight: 584 aa, 59 kDa
• Observed Molecular Weight: 59 kDa
• GenBank Accession Number: BC034697
• Gene Symbol: Cytokeratin 10
• Gene ID (NCBI): 3858
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P13645
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 10 (CK-10, K10, KRT10) is a type I (acidic) cytokeratin and part of the intermediate filament protein superfamily. It is expressed in keratinized stratified epithelium, suprabasal cell layers (including the stratum corneum), and differentiated areas of highly differentiated squamous cell carcinomas. CK-10 plays a role in maintaining the structural integrity of epithelial cells and is involved in differentiation, tissue specialization, and epidermal barrier establishment. Mutations in the KRT10 gene are associated with epidermolytic hyperkeratosis, and its expression is linked to the terminal differentiation of epidermal cells . CK-10 is also used as a marker for tissue differentiation and characterization of malignant tumors in surgical pathology.