Cytokeratin 19 Polyclonal antibody

Cytokeratin 19 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29855-1-AP

Synonyms

CK 19, CK19, Cytokeratin 19, K19, K1CS, keratin 19, KRT19



Tested Applications

Positive WB detected inBxPC-3 cells, HT-29 cells, HepG2 cells, MCF-7 cells, T-47D cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human thyroid cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29855-1-AP targets Cytokeratin 19 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 19 fusion protein Ag31469
Full Name keratin 19
Calculated Molecular Weight44KD
Observed Molecular Weight40-44 kDa
GenBank Accession NumberNM_002276
Gene Symbol KRT19
Gene ID (NCBI) 3880
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 19 is a type I cytokeratin. It is a biochemical marker of skin stem cells in vivo and in vitro.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IHC protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
IF protocol for Cytokeratin 19 antibody 29855-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols