Cytokeratin 24 Polyclonal antibody

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• Cytokeratin 24 Polyclonal antibody (33702-1-AP)

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  IHC staining of human breast cancer using 33702-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 33702-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 33702-1-AP (Cytokeratin 24 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 33702-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of A431 using 33702-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Cytokeratin 24 antibody (33702-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Cytokeratin 24 Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 33702-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

CK-24, Cytokeratin-24, K24, KA24, Keratin, type I cytoskeletal 24

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

33702-1-AP targets Cytokeratin 24 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag40474

  Product name: Recombinant human KRT24 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 426-525 aa of BC111968

  Sequence: AEYKQLLDIKTRLEVEIETYRRLLDGEGGGSSFAEFGGRNSGSVNMGSRDLVSGDSRSGSCSGQGRDSSKTRVTKTIVEELVDGKVVSSQVSSISEVKVK

• Full Name: keratin 24
• GenBank Accession Number: BC111968
• Gene Symbol: KRT24
• Gene ID (NCBI): 192666
• RRID: AB_3743055
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q2M2I5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 24 (CK-24), also known as Keratin 24 (KRT24), is a relatively recently identified type I cytokeratin. It is encoded by the KRT24 gene located on human chromosome 17. KRT24 is strongly expressed in keratinocytes, colon, spleen, and placenta. Previous research has suggested that KRT24 potentially acts as a oncogene in the early stages of colorectal cancer.