Cytokeratin 7 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 86153-7-RR (same clone as 86153-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86153-7-RR (CK7-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human appendicitis using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas using 86153-7-RR (same clone as 86153-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 86153-7-RR (Cytokeratin 7 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IF Staining of human breast cancer using 86153-7-RR (same clone as 86153-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human breast cancer tissue using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

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  IF Staining of HEPG2 using 86153-7-RR (same clone as 86153-7-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HEPG2 cells using Cytokeratin 7 antibody (86153-7-RR, Clone: 242229F1 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86153-7-PBS in a different storage buffer formulation.

Cytokeratin 7 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Cat No. 86153-7-PBS

Clone No.242229F1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Synonyms

KRT7, CK 7, CK7, CK-7, Cytokeratin,Keratin

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Product Information

86153-7-PBS targets Cytokeratin 7 in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 7
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_005556
• Gene Symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P08729
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Cytokeratin 7 (CK7) is a type II keratin protein that is a principal constituent of the intermediate filament cytoskeleton. It is primarily expressed in simple epithelia lining the cavities of internal organs, glandular ducts, and blood vessels. Abnormal expression of CK7 has been linked to various pathological conditions, including cancer. Overexpression of CK7 promotes tumor progression and metastasis in different human cancers, and its suppression leads to rapid tumor regression, highlighting its potential as a therapeutic target CK7 is also involved in inhibiting interferon-dependent interphase, promoting DNA synthesis, initiating translation possibly through interaction with p150 (the largest subunit of eukaryotic translation initiation factor 3), and interacting with G protein-coupled estrogen receptor 1 (GPER1), which activates several signaling pathways. In the context of cancer diagnosis, CK7 is used as a marker to distinguish between different types of carcinomas. It is expressed in most adenocarcinomas, particularly those of the lung and colorectal origin, and is useful in differentiating primary ovarian carcinoma from metastatic colorectal carcinoma. CK7 expression has also been studied as a predictor of an unfavorable prognosis in colorectal carcinoma.