Cytokeratin 74 Recombinant monoclonal antibody

• 

  WB analysis of mouse skin using 86325-2-RR

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 86325-2-RR (KRT74 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Cytokeratin 74 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 86325-2-RR

Clone No.250925D1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CK-74, Cytokeratin-74, K5C, K6IRS4, K74

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Tested Applications

• Positive WB detected in: mouse skin tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86325-2-RR targets Cytokeratin 74 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 74
• Observed Molecular Weight: 55-60 kDa
• GenBank Accession Number: BC160050
• Gene Symbol: KRT74
• Gene ID (NCBI): 121391
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7RTS7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 74 (KRT74) is a type II keratin protein, specifically found in the inner root sheath of hair follicles. It's crucial for hair formation and maintaining the structural integrity of hair. Mutations in the KRT74 gene can lead to various hair disorders, including woolly hair and hypotrichosis.