Cytokeratin 8 Monoclonal antibody

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  WB analysis of A549 using 60274-1-Ig

  A549 cells were subjected to SDS PAGE followed by western blot with 60274-1-Ig (Cytokeratin 8 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Cytokeratin 8 Monoclonal Antibody for WB, ELISA

Cat No. 60274-1-Ig

Clone No.2E10A10

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, ELISA

Synonyms

CARD2, Cell and organelle markers, CK 8, CK8, CYK8, Cytokeratin 8, Cytoskeleton Marker, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8

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Tested Applications

• Positive WB detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60274-1-Ig targets Cytokeratin 8 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: NM_002273
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_2881394
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P05787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT8 is often paired with keratin 18 in vivo.