Cytokeratin 8 Monoclonal antibody

Cytokeratin 8 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG3

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2E10A10

Cat no : 60274-1-Ig

Synonyms

CARD2, CK 8, CK8, CYK8, Cytokeratin 8, K2C8, K8, keratin 8, KO, KRT8, Type II keratin Kb8



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman stomach tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

60274-1-Ig targets Cytokeratin 8 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name keratin 8
Calculated molecular weight 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberNM_002273
Gene symbol KRT8
Gene ID (NCBI) 3856
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9–K23, and the hair keratins Ha1–Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1–K8, and the hair keratins, Hb1–Hb6. KRT8 is often paired with keratin 18 in vivo.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 8 antibody 60274-1-IgDownload protocol
IHC protocol for Cytokeratin 8 antibody 60274-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols