Cytokeratin 8 Monoclonal antibody

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• Cytokeratin 8 Monoclonal antibody (68627-5-Ig)

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  IHC staining of human hepatocirrhosis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human hepatocirrhosis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 68627-5-Ig

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 68627-5-Ig (Cytokeratin 8 antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 8 Monoclonal Antibody for IHC, ELISA

Cat No. 68627-5-Ig

Clone No.2D9G8

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IHC, ELISA

Synonyms

KRT8, CARD2, CK 8, CK8, CK-8

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Tested Applications

• Positive IHC detected in: human hepatocirrhosis tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:5000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68627-5-Ig targets Cytokeratin 8 in IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0488

  Product name: Recombinant human KRT8 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-483 aa of BC000654

  Sequence: MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK

• Full Name: keratin 8
• Calculated Molecular Weight: 54 kDa
• GenBank Accession Number: BC000654
• Gene Symbol: Cytokeratin 8
• Gene ID (NCBI): 3856
• RRID: AB_3743310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P05787
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cytokeratin 8 (CK8) is a protein that belongs to the cytokeratin family, which is a group of structural proteins found in the intracytoplasmic cytoskeleton of epithelial cells. CK8 forms intermediate filaments within the cytoplasm of simple epithelial cells, typically as a dimer with cytokeratin 18 (CK18). These filaments provide a stabilizing framework that helps determine cell shape and enables cells to cope with mechanical stress. CK8/CK18 filaments also serve as a mesh of "paths" for signaling molecules, metabolites, and pathogens to travel within the cell in an organized manner. CK8 is expressed in various epithelial tissues and is particularly associated with glandular and simple epithelia. Its expression is also a significant marker in several types of cancer, including lung and esophageal cancer, where it can indicate tumor progression and poor prognosis.