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  • KD/KO Validated

D2HGDH Polyclonal antibody

D2HGDH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF

Conjugate

Unconjugated

Cat no : 13895-1-AP

Synonyms

D2HGD, D2HGDH



Tested Applications

Positive WB detected inmouse kidney tissue, human liver tissue, human heart tissue, rat liver tissue, mouse liver tissue
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13895-1-AP targets D2HGDH in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen D2HGDH fusion protein Ag4857
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated molecular weight 521 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC036604
Gene symbol D2HGDH
Gene ID (NCBI) 728294
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for D2HGDH antibody 13895-1-APDownload protocol
IHC protocol for D2HGDH antibody 13895-1-APDownload protocol
IF protocol for D2HGDH antibody 13895-1-APDownload protocol
IP protocol for D2HGDH antibody 13895-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Hypoxia Induces Production of L-2-Hydroxyglutarate.

Authors - Andrew M Intlekofer
  • KD Validated
humanWB

Cell Metab

Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.

Authors - William M Oldham
  • KD Validated
humanWB, IF

Nat Commun

D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.

Authors - An-Ping Lin
humanWB

Leukemia

MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.

Authors - An-Ping Lin
WB

Oncotarget

D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.

Authors - Shenghong Ma

Hum Mutat

D-2-hydroxyglutaric aciduria Type I: Functional analysis of D2HGDH missense variants.

Authors - Ana Pop