Validation Data Gallery
|Positive WB detected in||mouse kidney tissue, human liver tissue, human heart tissue, rat liver tissue, mouse liver tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13895-1-AP targets D2HGDH in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||D2HGDH fusion protein Ag4857|
|Full Name||D-2-hydroxyglutarate dehydrogenase|
|Calculated molecular weight||521 aa, 56 kDa|
|Observed molecular weight||56 kDa|
|GenBank accession number||BC036604|
|Gene ID (NCBI)||728294|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).It has 2 isoforms produced by alternative splicing.
|Product Specific Protocols|
|WB protocol for D2HGDH antibody 13895-1-AP||Download protocol|
|IHC protocol for D2HGDH antibody 13895-1-AP||Download protocol|
|IF protocol for D2HGDH antibody 13895-1-AP||Download protocol|
|IP protocol for D2HGDH antibody 13895-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Hypoxia Induces Production of L-2-Hydroxyglutarate.
Hypoxia-Mediated Increases in L-2-hydroxyglutarate Coordinate the Metabolic Response to Reductive Stress.
D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2.
MYC, mitochondrial metabolism and O-GlcNAcylation converge to modulate the activity and subcellular localization of DNA and RNA demethylases.
D-2-hydroxyglutarate is essential for maintaining oncogenic property of mutant IDH-containing cancer cells but dispensable for cell growth.
D-2-hydroxyglutaric aciduria Type I: Functional analysis of D2HGDH missense variants.