DAAM1 Polyclonal antibody

• Featured Product
• KD/KO Validated

DAAM1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

21

Reviews (3)

Cat no : 14876-1-AP

Synonyms

DAAM1, KIAA0666

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Validation Data Gallery

• 

  WB analysis of mouse brain using 14876-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 14876-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 14876-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14876-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14876-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14876-1-AP (DAAM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 14876-1-AP

  IP result of anti-DAAM1 (IP:14876-1-AP, 3ug; Detection:14876-1-AP 1:500) with mouse liver tissue lysate 8000ug.

• 

  IHC staining of human brain using 14876-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14876-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14876-1-AP (DAAM1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of HepG2 using 14876-1-AP

  Immunofluorescent analysis of HepG2 cells, using DAAM1 antibody 14876-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue
• Positive IP detected in: mouse liver tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 16 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Product Information

14876-1-AP targets DAAM1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, Xenopus, Canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAAM1 fusion protein Ag6648
• Full Name: dishevelled associated activator of morphogenesis 1
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 123 kDa
• GenBank Accession Number: BC064999
• Gene Symbol: DAAM1
• Gene ID (NCBI): 23002
• RRID: AB_2089444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.

Publications

Species	Application	Title
human	WB,IF	J Cell Biol; DAAM1 stabilizes epithelial junctions by restraining WAVE complex-dependent lateral membrane motility.; Authors - Tamako Nishimura ; KD Validated
mouse	WB,IHC	Development; Dishevelled-associated activator of morphogenesis 1 (Daam1) is required for heart morphogenesis.; Authors - Li Deqiang D ; KO Validated
human	WB,IF,IHC	Cell Prolif; Formin homology domains of Daam1 bind to Fascin and collaboratively promote pseudopodia formation and cell migration in breast cancer.; Authors - Leiyu Hao
human	WB	Cell Signal; miR-613 inhibits cell migration and invasion by downregulating Daam1 in triple-negative breast cancer.; Authors - Huaping Xiong
human	WB	Mol Biol Cell; Formin-dependent TGF-β signaling for epithelial to mesenchymal transition.; Authors - Manish K Rana
human	WB	Am J Physiol Cell Physiol; Secreted Wnt6 mediates diabetes-associated centrosome amplification via its receptor FZD4.; Authors - Qin Ju He

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Vanina (Verified Customer) (11-11-2020)

Cell staining using 1:100 antibody dilution

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: MDA-MB-361 breat cancer cell line

DAAM1 Antibody Immunofluorescence validation (1:100 dilution) in MDA-MB-361 breat cancer cell line (Cat no:14876-1-AP)

FH

Vanina (Verified Customer) (11-11-2020)

Western blot using 20 µg of protein per lane.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: MDA-MB-231 and MDA-MB-453 breast cancer cell lines

DAAM1 Antibody Western Blot validation (1:1000 dilution) in MDA-MB-231 and MDA-MB-453 breast cancer cell lines (Cat no:14876-1-AP)

FH

Tongbin (Verified Customer) (08-25-2020)

Although I didn't see any differences in Daam1 protein level between our wild type and knockout animals. This antibody was very specific and had a sharp ~125kD band.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart

DAAM1 Antibody Western Blot validation (1:1000 dilution) in Heart (Cat no:14876-1-AP)

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