Tested Applications
| Positive WB detected in | mouse brain tissue, HepG2 cells, human heart tissue, mouse liver tissue, mouse heart tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human brain tissue, human placenta tissue, mouse cerebellum tissue, rat cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14876-1-AP targets DAAM1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, canine, goat, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6648 Product name: Recombinant human DAAM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 715-1068 aa of BC064999 Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF Predict reactive species |
| Full Name | dishevelled associated activator of morphogenesis 1 |
| Calculated Molecular Weight | 123 kDa |
| Observed Molecular Weight | 123 kDa |
| GenBank Accession Number | BC064999 |
| Gene Symbol | DAAM1 |
| Gene ID (NCBI) | 23002 |
| RRID | AB_2089444 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4D1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IHC protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| IP protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| WB protocol for DAAM1 antibody 14876-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DAAM1 Polyclonal antibody (catalog number 14876-1-AP) has 34 citations. These appear in journals including Science, Cancer Cell, Signal Transduction and Targeted Therapy, Molecular Cancer, Advanced Science, Cell Reports, Cell Death & Discovery, Development, Journal of Cell Biology, Molecular Biology of the Cell, PLoS One, and Oncology Targets & Therapy. Research fields span cancer biology (breast, lung, gastric, ovarian, pancreatic cancers), cell migration and invasion, Wnt/PCP signaling, cytoskeletal remodeling, development (nephron, heart, follicle), immunotherapy, and cardiovascular disease.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther C/EBPβ activation in vascular smooth muscle cells promotes hyperlipidemia-induced phenotypic transition and arterial stiffness | ||
Science The membrane skeleton is constitutively remodeled in neurons by calcium signaling. | ||
Mol Cancer Hsa_circ_0125356 promotes gemcitabine resistance by modulating WNT canonical and non-canonical pathways via miR-582-5p/FGF9 axis in non-small cell lung cancer | ||
Bioact Mater Mesenchymal stromal/stem cell spheroid-derived extracellular vesicles advance the therapeutic efficacy of 3D-printed vascularized artificial liver lobules in liver failure treatment | ||
Adv Sci (Weinh) Polycystin-1 Mutant Alters Mechanotransduction in Response to Collagen and Extracellular Matrix Stiffness via Daam1-Dependent Microfilament Remodeling |
Reviews
What customers say
All three reviews rated the antibody 5 stars. Users reported strong performance in both Western Blot (1:1000 dilution) and Immunofluorescence (1:100 dilution) across breast cancer cell lines and heart tissue. One user highlighted the antibody's high specificity, noting a sharp ~125 kD band, though they observed no difference between wild-type and knockout samples. Overall, reviewers found the antibody reliable, specific, and well-suited for detecting the target at the expected molecular weight.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Vanina (Verified Customer) (11-11-2020) | Cell staining using 1:100 antibody dilution
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Vanina (Verified Customer) (11-11-2020) | Western blot using 20 µg of protein per lane.
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Tongbin (Verified Customer) (08-25-2020) | Although I didn't see any differences in Daam1 protein level between our wild type and knockout animals. This antibody was very specific and had a sharp ~125kD band.
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