DAAM1 Monoclonal antibody, PBS Only

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  WB analysis using 67287-1-Ig (same clone as 67287-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat heart using 67287-1-Ig (same clone as 67287-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse heart using 67287-1-Ig (same clone as 67287-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 67287-1-Ig (DAAM1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human skin cancer using 67287-1-Ig (same clone as 67287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67287-1-Ig (DAAM1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67287-1-PBS in a different storage buffer formulation.

DAAM1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 67287-1-PBS

Clone No.3F11F11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

3F11F11, Disheveled-associated activator of morphogenesis 1, FLJ41657, KIAA0666

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Product Information

67287-1-PBS targets DAAM1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6809

  Product name: Recombinant human DAAM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 715-1068 aa of BC064999

  Sequence: KDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF

• Full Name: dishevelled associated activator of morphogenesis 1
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC064999
• Gene Symbol: DAAM1
• Gene ID (NCBI): 23002
• RRID: AB_2882553
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9Y4D1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Dishevelled-associated activator of morphogenesis 1 (Daam1) is a member of the formin protein family that is implicated in actin assembly. Daam1 also plays a critical role in mediating Wnt/Fz signaling pathway and regulates cell polarity during development and tissue homoeostasis. This antibody well recognizes the endogenous DAAM1 protein around 120 kDa.