Product Information
The HRP enzyme-labelled anti-mouse/rabbit secondary antibody binds specifically to the heavy and light chains of mouse and rabbit IgG. It passes through the diaminobenzidine (DAB) chromogenic solution to form a tan insoluble product. It is mainly used for immunohistochemical staining.
Components | Size | |
Solution A | 20 mL | 100 mL |
Solution B | 1 mL | 5 mL |
Storage
Store at 2-8℃ away from light, valid for 12 months.
Usage
1. DAB Staining Solution (freshly prepared as required): Mix solution A and solution B in a dilution ratio of 20:1, and use.
2. Add 50-100 uL of the above DAB staining solution to completely cover the sectioned tissue (if the tissue is large, the amount can be increased appropriately), incubate for 3-10 minutes, rinse with distilled water, and terminate the colour development.
Cautions
1. This product is used in immunohistochemistry experiments only.
2. Appropriate precautions should be taken to avoid the reagents' contact with skin and eyes.
3. This product is for scientific research use only. If you need to use it for diagnosis, please contact us for the record of product.
Cited in Article as
PR30018, DAB kit (IHC), Proteintech, IL, USA
Publications
| Application | Title |
|---|---|
Drug Resist Updat AMPKα2 attenuates doxorubicin induced ferroptosis by promoting NCOA4 degradation in triple negative breast cancer. | |
Bone Res NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling | |
Nat Commun C2orf74 orchestrates germ-Leydig crosstalk to inhibit white adipose tissue browning in male mice. | |
Int J Oral Sci Periodontitis pathogen Porphyromonas gingivalis promotes chronic obstructive pulmonary disease via affecting neutrophils chemotaxis and function. | |
J Adv Res Restoring cardiolipin homeostasis mitigates cerebral ischemia-reperfusion injury by suppressing ATG5-mediated neuronal autophagy-dependent ferroptosis. | |
Acta Pharm Sin B Celastrol directly targets LRP1 to inhibit fibroblast-macrophage crosstalk and ameliorates psoriasis progression |
