DAB kit (IHC)

Cat No. PR30018
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Product Information

The HRP enzyme-labelled anti-mouse/rabbit secondary antibody binds specifically to the heavy and light chains of mouse and rabbit IgG. It passes through the diaminobenzidine (DAB) chromogenic solution to form a tan insoluble product.  It is mainly used for immunohistochemical staining.

Components

Size

Solution A

20 mL

100 mL

Solution B

1 mL

5 mL


Storage

Store at 2-8℃ away from light, valid for 12 months.

Usage

1. DAB Staining Solution (freshly prepared as required): Mix solution A and solution B in a dilution ratio of 20:1, and use.

2. Add 50-100 uL of the above DAB staining solution to completely cover the sectioned tissue (if the tissue is large, the amount can be increased appropriately), incubate for 3-10 minutes, rinse with distilled water, and terminate the colour development.

Cautions

1. This product is used in immunohistochemistry experiments only.

2. Appropriate precautions should be taken to avoid the reagents' contact with skin and eyes.

3. This product is for scientific research use only. If you need to use it for diagnosis, please contact us for the record of product.

Cited in Article as

PR30018, DAB kit (IHC), Proteintech, IL, USA

Publications

ApplicationTitle

J Ethnopharmacol

Inhibition of Bcl-2/Bax/cleaved caspase-3 pathway activity by rare ginsenosides ameliorates cyclophosphamide-induced oligoasthenospermia (OA) in male mice

Authors - Fei Yan

J Gastrointest Oncol

A novel lactylation-related signature for predicting esophageal cancer prognosis and immune infiltration

Authors - Liming Xie

RSC Adv

Novel crosslinked recombinant human collagen scaffolds with excellent cytocompatibility and wound healing effect

Authors - Yue He

Cell Death Discov

NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation

Authors - Zhiming Liu

Vet Res

GHSR gene knockout alleviates the liver pathological response in Echinococcus granulosus infection by reducing parasite survival

Authors - Jiang Zhu

Acta Pharm Sin B

Celastrol directly targets LRP1 to inhibit fibroblast-macrophage crosstalk and ameliorates psoriasis progression

Authors - Yuyu Zhu
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