DAB kit (IHC)

• 

  Immunohistochemistry of 84192-4-RR (Ki67 rabbit recombinant antibody, 1:4000) in human tonsillitis tissue. PR30018 (DAB) was used as substrate.

• 

  Immunohistochemistry of 68218-1-Ig (CD163 mouse monoclonal antibody, 1:5000) in human placenta tissue. PR30018 (DAB) was used as substrate.

• 

  Immunohistochemistry of 67793-1-Ig (Desmin mouse monoclonal antibody, 1:2000) in human placenta tissue. PR30018 (DAB) was used as substrate.

• 

  Immunohistochemistry of 80232-1-RR (Vim rabbit recombinant antibody, 1:5000) in human placenta tissue. PR30018 (DAB) was used as substrate.

• 

  Immunohistochemistry of 68377-1-Ig (CEA mouse monoclonal antibody, 1:2000) in human rectal cancer tissue. PR30018 (DAB) was used as substrate.

Cat No. PR30018

Publications

8

---

Product Information

The HRP enzyme-labelled anti-mouse/rabbit secondary antibody binds specifically to the heavy and light chains of mouse and rabbit IgG. It passes through the diaminobenzidine (DAB) chromogenic solution to form a tan insoluble product.  It is mainly used for immunohistochemical staining.

Components	Size
Solution A	20 mL	100 mL
Solution B	1 mL	5 mL

Storage

Store at 2-8℃ away from light, valid for 12 months.

Usage

1. DAB Staining Solution (freshly prepared as required): Mix solution A and solution B in a dilution ratio of 20:1, and use.

2. Add 50-100 uL of the above DAB staining solution to completely cover the sectioned tissue (if the tissue is large, the amount can be increased appropriately), incubate for 3-10 minutes, rinse with distilled water, and terminate the colour development.

Cautions

1. This product is used in immunohistochemistry experiments only.

2. Appropriate precautions should be taken to avoid the reagents' contact with skin and eyes.

3. This product is for scientific research use only. If you need to use it for diagnosis, please contact us for the record of product.

Cited in Article as

PR30018, DAB kit (IHC), Proteintech, IL, USA

Publications

Application	Title
IHC	Sci Rep; Modulation of the tumor immune microenvironment by Interferon Regulatory Factor 8 enhances immunotherapy in lung adenocarcinoma; Authors - Wen Huo
IHC	Bone Res; NELL2, a novel osteoinductive factor, regulates osteoblast differentiation and bone homeostasis through fibronectin 1/integrin-mediated FAK/AKT signaling; Authors - Hairui Yuan
	Cell Death Discov; NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation; Authors - Zhiming Liu
	J Agric Food Chem; Nuciferine Restores Autophagy via the PI3K-AKT-mTOR Pathway to Alleviate Renal Fibrosis in Diabetic Kidney Disease; Authors - Tongqing Li
	Vet Res; GHSR gene knockout alleviates the liver pathological response in Echinococcus granulosus infection by reducing parasite survival; Authors - Jiang Zhu
	Acta Pharm Sin B; Celastrol directly targets LRP1 to inhibit fibroblast-macrophage crosstalk and ameliorates psoriasis progression; Authors - Yuyu Zhu