DAB2IP Polyclonal antibody

• 

  WB analysis using 23582-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23582-1-AP (DAB2IP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 23582-1-AP

  IP result of anti-DAB2IP (IP:23582-1-AP, 4ug; Detection:23582-1-AP 1:3000) with mouse brain tissue lysate 1280 ug.

DAB2IP Polyclonal Antibody for WB, IP, ELISA

Cat No. 23582-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

AF9Q34, AIP1, ASK interacting protein 1, DAB2 interacting protein, DAB2 interaction protein, DAB2IP, DIP1/2, KIAA1743

---

Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells, mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

23582-1-AP targets DAB2IP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20308

  Product name: Recombinant human DAB2IP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 523-700 aa of BC146762

  Sequence: RDVHTALSTPGSGQLPGTNDLASTPGSGSSSISAGLQKMVIENDLSGLIDFTRLPSPTPENKDLFFVTRSSGVQPSPARSSSYSEANEPDLQMANGGKSLSMVDLQDARTLDGEAGSPAGPDVLPTDGQAAAAQLVAGWPARATPVNLAGLATVRRAGQTPTTPGTSEGAPGRPQLLA

• Full Name: DAB2 interacting protein
• Calculated Molecular Weight: 1189 aa, 132 kDa
• Observed Molecular Weight: 118 kDa
• GenBank Accession Number: BC146762
• Gene Symbol: DAB2IP
• Gene ID (NCBI): 153090
• RRID: AB_2879300
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q5VWQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB,IHC	CNS Neurosci Ther; Integration of Methylation and Gene Expression Deciphered Candidate Biomarkers DAB2IP and SMYD3 in Delayed Encephalopathy After Carbon Monoxide Poisoning; Authors - Hongyi Yan
human	WB, IF, IHC	Cancer Sci; DOC-2/DAB2 interactive protein destabilizes c-Myc to impair the growth and self-renewal of colon tumor-repopulating cells.; Authors - Haiou Li
human	WB,IHC	Biochim Biophys Acta Mol Basis Dis; DNMT3A facilitates colorectal cancer progression via regulating DAB2IP mediated MEK/ERK activation.; Authors - Yunjiao Zhou
Mouse		Toxicol Res (Camb); Cypermethrin inhibits proliferation of Sertoli cells through AR involving DAB2IP/PI3K/AKT signaling pathway in vitro; Authors - Qi Wang
human	WB,IHC,IF	Cell Death Dis; Soft fibrin matrix downregulates DAB2IP to promote Nanog-dependent growth of colon tumor-repopulating cells.; Authors - Meng Zhang
human	WB	Mol Cell; The RNA-stability-independent role of the RNA m6A reader YTHDF2 in promoting protein translation to confer tumor chemotherapy resistance; Authors - Tao Liu