DALRD3 Polyclonal antibody

DALRD3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26294-1-AP

Synonyms

DALRD3

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Validation Data Gallery

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  WB analysis of COLO 320 using 26294-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 26294-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 26294-1-AP (DALRD3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 26294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 26294-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26294-1-AP (DALRD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, COLO 320 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

26294-1-AP targets DALRD3 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DALRD3 fusion protein Ag23437
• Full Name: DALR anticodon binding domain containing 3
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC047683
• Gene Symbol: DALRD3
• Gene ID (NCBI): 55152
• RRID: AB_2880467
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; DALRD3 encodes a protein mutated in epileptic encephalopathy that targets arginine tRNAs for 3-methylcytosine modification.; Authors - Jenna M Lentini