DAND5 Polyclonal antibody

DAND5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22137-1-AP

Synonyms

CER2, Cerberus like protein 2, Cerl 2, CERL2, CKTSF1B3, COCO, CRL2, DAN domain family member 5, DAN domain family, member 5, DAND5, DANTE, GREM3, Gremlin 3, SP1

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Validation Data Gallery

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  WB analysis of human heart using 22137-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22137-1-AP (DAND5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 22137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

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  IHC staining of human testis using 22137-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 22137-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 22137-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart slide using 22137-1-AP (DAND5 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IHC detected in: human testis tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22137-1-AP is DAND5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAND5 fusion protein Ag17533
• Full Name: DAN domain family, member 5
• Calculated Molecular Weight: 189 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC113476
• Gene Symbol: DAND5
• Gene ID (NCBI): 199699
• RRID: AB_2879004
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.