DAP3 Polyclonal antibody

DAP3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10276-1-AP

Synonyms

MRPS29, MRP S29, Death-associated protein 3, DAP-3, DAP 3

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Validation Data Gallery

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  WB analysis of HeLa using 10276-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10276-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10276-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10276-1-AP (DAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 10276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 10276-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10276-1-AP (DAP3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue
• Positive IHC detected in: human stomach tissue, human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

10276-1-AP targets DAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAP3 fusion protein Ag0274
• Full Name: death associated protein 3
• Calculated Molecular Weight: 46 kDa
• Observed Molecular Weight: 40-45 kDa
• GenBank Accession Number: BF220193
• Gene Symbol: DAP3
• Gene ID (NCBI): 7818
• RRID: AB_10695891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
mouse	WB	Noncoding RNA Res; DNA methylation of microRNA-365-1 induces apoptosis of hair follicle stem cells by targeting DAP3; Authors - Xin Liu