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  • KD/KO Validated

DAPK1 Polyclonal antibody

DAPK1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25136-1-AP

Synonyms

DAP kinase 1, DAPK, DAPK1



Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 25136-1-AP is DAPK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity goat, human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag18387
Full Name death-associated protein kinase 1
Calculated molecular weight 1430 aa, 160 kDa
Observed molecular weight 140-160 kDa
GenBank accession numberBC113660
Gene symbol DAPK1
Gene ID (NCBI) 1612
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for DAPK1 antibody 25136-1-APDownload protocol
IHC protocol for DAPK1 antibody 25136-1-APDownload protocol
IF protocol for DAPK1 antibody 25136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB, IF, IHC

Aging (Albany NY)

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Authors - Zhengshuai Song
ratWB

Cells Tissues Organs

Death-associated Protein Kinase 1 Regulates Oxidative Stress In Cardiac Ischemia Reperfusion Injury.

Authors - Wentong Li
  • KD Validated
goatWB

Animals (Basel)

Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats.

Authors - Zhiwei Kong
humanWB,IHC

Stem Cells Dev

Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Authors - Shaojun Nong
  • KD Validated
mouseWB

J Alzheimers Dis

Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.

Authors - Hasan A M M Almansoub
mouseWB

J Biol Chem

PMID: 28174303

Authors - Barbara Kroczynska