|Positive WB detected in||A549 cells|
|Positive IHC detected in||human small intestine tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:100-1:400|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 25136-1-AP is DAPK1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, mouse, rat, goat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DAPK1 fusion protein Ag18387|
|Full Name||death-associated protein kinase 1|
|Calculated molecular weight||1430 aa, 160 kDa|
|Observed molecular weight||140-160 kDa|
|GenBank accession number||BC113660|
|Gene ID (NCBI)||1612|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.
Stem Cell Res Ther
Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.
J Biol Chem
J Alzheimers Dis
Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.
Stem Cells Dev
Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.
Comparison of the Effects of Nonprotein and Protein Nitrogen on Apoptosis and Autophagy of Rumen Epithelial Cells in Goats.
Cells Tissues Organs
Death-associated Protein Kinase 1 Regulates Oxidative Stress In Cardiac Ischemia Reperfusion Injury.