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  • KD/KO Validated

DAPK1 Polyclonal antibody

DAPK1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 25136-1-AP


DAP kinase 1, DAPK, DAPK1

Tested Applications

Positive WB detected inA549 cells
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25136-1-AP is DAPK1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAPK1 fusion protein Ag18387
Full Name death-associated protein kinase 1
Calculated Molecular Weight 1430 aa, 160 kDa
Observed Molecular Weight 140-160 kDa
GenBank Accession NumberBC113660
Gene Symbol DAPK1
Gene ID (NCBI) 1612
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAPK1(Death-associated protein kinase 1) is a stress-activated tumor suppressor protein that plays a role in both proapoptotic or antiapoptotic signal transduction pathways. Loss of DAPK1 expression is associated with a selective advantage fortumor cells to resist apoptotic stimuli, allowing them to separate from the original tumor; from this point of view, DAPK1 could be considered a tumor metastases inhibitor gene(PMID:17319784). This protein has 3 isoforms produced by alternative splicing.


Product Specific Protocols
WB protocol for DAPK1 antibody 25136-1-APDownload protocol
IHC protocol for DAPK1 antibody 25136-1-APDownload protocol
IF protocol for DAPK1 antibody 25136-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Life Sci

Auto- and paracrine rewiring of NIX-mediated mitophagy by insulin-like growth factor-binding protein 7 in septic AKI escalates inflammation-coupling tubular damage

Authors - Bang-Chuan Hu
humanWB, IF, IHC

Aging (Albany NY)

Low DAPK1 expression correlates with poor prognosis and sunitinib resistance in clear cell renal cell carcinoma.

Authors - Zhengshuai Song

Stem Cell Res Ther

Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.

Authors - Mengqi Chu
  • KD Validated

J Biol Chem

PMID: 28174303

Authors - Barbara Kroczynska

J Alzheimers Dis

Oxytocin Alleviates MPTP-Induced Neurotoxicity in Mice by Targeting MicroRNA-26a/Death-Associated Protein Kinase 1 Pathway.

Authors - Hasan A M M Almansoub

Stem Cells Dev

Reduced DAPK1 Expression Promotes Stem Cell-Like Characteristics of Prostate Cancer Cells by Activating ZEB1 via Hippo/YAP Signaling Pathway.

Authors - Shaojun Nong
  • KD Validated