Multi-rAb® DARS2 Multi-Recombinant antibody

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  WB analysis using RMX00040

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00040 (DARS2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using RMX00040

  IP result of anti-DARS2 (IP:RMX00040, 4ug; Detection:RMX00040 1:10000) with A549 cells lysate 920 ug.

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  IHC staining of human gliomas using RMX00040

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using RMX00040 (DARS2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using RMX00040

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using RMX00040 (DARS2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using RMX00040

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using DARS2 antibody (RMX00040) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of A431 using RMX00040

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using DARS2 antibody (RMX00040) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

DARS2 Multi-Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. RMX00040

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Aspartate  tRNA ligase, Aspartate--tRNA ligase, mitochondrial, Aspartyl-tRNA synthetase, AspRS, EC:6.1.1.12

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Tested Applications

• Positive WB detected in: A549 cells, K-562 cells, MCF-7 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00040 targets DARS2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: aspartyl-tRNA synthetase 2, mitochondrial
• Calculated Molecular Weight: 645 aa, 74 kDa
• Observed Molecular Weight: 66-74 kDa
• GenBank Accession Number: BC045173
• Gene Symbol: DARS2
• Gene ID (NCBI): 55157
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q6PI48
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DARS2 is also named as AspRS (aspartyl-tRNA synthetase) and belongs to the class-II aminoacyl-tRNA synthetase family. The deduced 645-amino acid protein has a 47-amino acid mitochondrial targeting signal, resulting in a mature protein of 598 amino acids. DARS2 contains conserved residues involved in ATP binding, tRNA binding, and aspartic acid recognition, as well as catalytic site motifs characteristic of amino acid tRNA synthetases. It is a dimeric proteins(PMID:19443655). Rat aspartyl-tRNA synthetase has a N-terminal polypeptide extension of about 40 amino acid residues which can be removed without impairing its catalytic activity(PMID:9030747). This protein has different molecular mass(55 kDa, 50 kDa,66 kDa ) according to the publications(PMID:11306575; 19443655;15299749).