DAZ4 Polyclonal antibody

DAZ4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14065-1-AP

Synonyms

pDP1681, pDP1680, DAZ1, DAZ 4, DAZ

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Validation Data Gallery

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  WB analysis of mouse testis using 14065-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat testis using 14065-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14065-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 14065-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 14065-1-AP (DAZ4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 14065-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14065-1-AP (DAZ4 antibody) at dilution of 1:120 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, human testis tissue, rat testis tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

14065-1-AP targets DAZ4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DAZ4 fusion protein Ag5116
• Full Name: deleted in azoospermia 4
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: BC047617
• Gene Symbol: DAZ4
• Gene ID (NCBI): 57135
• RRID: AB_2878008
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAZ4, encoded by the DAZ gene, is an RNA-binding protein with a role in spermatogenesis. DAZ4 is a testis-specific protein that expression restricted to premeiotic germ cells, particularly in spermatogonia. Defects in DAZ4 may be a cause of spermatogenic failure Y-linked type 2 (SPGFY2). It is a disorder resulting in the absence (azoospermia) or reduction (oligozoospermia) of sperm in the semen, leading to male infertility.