DAZL Polyclonal antibody

DAZL Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 12633-1-AP

Synonyms

DAZ homolog, DAZ like autosomal, DAZH, DAZL, DAZL1, DAZLA, deleted in azoospermia like, Deleted in azoospermia like 1, SPGY like autosomal, SPGYLA



Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inmouse testis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse testis tissue
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12633-1-AP targets DAZL in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DAZL fusion protein Ag3326
Full Name deleted in azoospermia-like
Calculated molecular weight 295 aa, 33 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC027595
Gene symbol DAZL
Gene ID (NCBI) 1618
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DAZL antibody 12633-1-APDownload protocol
IHC protocol for DAZL antibody 12633-1-APDownload protocol
IF protocol for DAZL antibody 12633-1-APDownload protocol
IP protocol for DAZL antibody 12633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB

Proteomics

Systematic in-depth proteomic analysis of mitochondria-associated endoplasmic reticulum membranes (MAM) in mouse and human testes.

Authors - Xiaoli Wang
ratIF

Stem Cell Res Ther

Concentrated exosomes from menstrual blood-derived stromal cells improves ovarian activity in a rat model of premature ovarian insufficiency.

Authors - Siwen Zhang