Multi-rAb® DAZL Multi-Recombinant antibody

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• Multi-rAb® DAZL Multi-Recombinant antibody (RMX00077)

DAZL Multi-Recombinant Antibody for WB, IHC, IF-P, ELISA

Cat No. RMX00077

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

DAZH, DAZL1, DAZLA, DAZ-like autosomal, Deleted in azoospermia-like

• 

  WB analysis using RMX00077

  Various lysates were subjected to SDS PAGE followed by western blot with RMX00077 (DAZL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using RMX00077

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00077 (DAZL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat testis using RMX00077

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using RMX00077 (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using RMX00077

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00077 (DAZL antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse testis using RMX00077

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using RMX00077 (DAZL antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using RMX00077

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (RMX00077) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse testis using RMX00077

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using DAZL antibody (RMX00077) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse testis tissue, rat testis tissue
• Positive IHC detected in: mouse testis tissue, rat testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

RMX00077 targets DAZL in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Multi-Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: deleted in azoospermia-like
• Calculated Molecular Weight: 295 aa, 33 kDa
• Observed Molecular Weight: 37 kDa
• GenBank Accession Number: BC027595
• Gene Symbol: DAZL
• Gene ID (NCBI): 1618
• RRID: AB_3750126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: N/A
• UNIPROT ID: Q92904
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DAZL proteins are germ cell-specific RNA-binding proteins and are considered major regulators of spermatogenesis. Because DAZL is a novel protein expressed specifically in germ cells, it is widely employed as a germ cell marker. In humans, immunostaining shows DAZL is abundant in the cytoplasm of primary spermatocytes, but scarce in spermatogonia.