DBP Polyclonal antibody

• 

  WB analysis using 12662-1-AP

  SH-SY5Y cells lysates were subjected to SDS PAGE followed by western blot with 12662-1-AP (DBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 12662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12662-1-AP (DBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12662-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12662-1-AP (DBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

DBP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 12662-1-AP

Publications(12)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

Albumin D box binding protein, D site binding protein, DABP, DBP, TaxREB302

---

Tested Applications

• Positive WB detected in: SH-SY5Y cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Product Information

12662-1-AP targets DBP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3343

  Product name: Recombinant human DBP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-325 aa of BC011965

  Sequence: MARPVSDRTPAPLLLGGPAGTPPGGGALLGLRSLLQGTSKPKEPASCLLKEKERKAALPAATTPGPGLETAGPADAPAGAVVGGGSPRGRPGPVPAPGLLAPLLWERTLPFGDVEYVDLDAFLLEHGLPPSPPPPGGPSPEPSPARTPAPSPGPGSCGSASPRSSPGHAPARAALGTASGHRAGLTSRDTPSPVDPDTVEVLMTFEPDPADLALSSIPGHETFDPRRHRFSEEELKPQPIMKKARKIQVPEEQKDEKYWSRRYKNNEAAKRSRDARRLKENQISVRAAFLEKENALLRQEVVAVRQELSHYRAVLSRYQAQHGAL

• Full Name: D site of albumin promoter (albumin D-box) binding protein
• Calculated Molecular Weight: 325 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC011965
• Gene Symbol: DBP
• Gene ID (NCBI): 1628
• RRID: AB_2877870
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q10586
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	Sci Adv; Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell function.; Authors - Matthew R Brown
mouse	WB	Theranostics; PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.; Authors - Shuai Wang
mouse	WB	FASEB J; Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver.; Authors - Wenxiang Zhang
mouse	WB	Am J Physiol Gastrointest Liver Physiol; Parenteral nutrition results in peripheral ileal to hepatic circadian discordance in mice; Authors - Colin T Shearn
mouse	WB	Commun Biol; ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude.; Authors - Huilin Li
rat,human	WB,IHC	Sci Rep; Gene expression profiles during tissue remodeling following bladder outlet obstruction.; Authors - Saya Ito ; KD Validated