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  • KD/KO Validated

DBP Polyclonal antibody

DBP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12662-1-AP

Synonyms

Albumin D box binding protein, D site binding protein, DABP, DBP, TaxREB302



Tested Applications

Positive WB detected inSH-SY5Y cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12662-1-AP targets DBP in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DBP fusion protein Ag3343
Full Name D site of albumin promoter (albumin D-box) binding protein
Calculated Molecular Weight 325 aa, 34 kDa
Observed Molecular Weight34 kDa
GenBank Accession NumberBC011965
Gene Symbol DBP
Gene ID (NCBI) 1628
RRIDAB_2877870
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DBP antibody 12662-1-APDownload protocol
IHC protocol for DBP antibody 12662-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Sci Adv

Time-restricted feeding prevents deleterious metabolic effects of circadian disruption through epigenetic control of β cell function.

Authors - Matthew R Brown
mouseWB

Theranostics

PPAR-γ integrates obesity and adipocyte clock through epigenetic regulation of Bmal1.

Authors - Shuai Wang
mouseWB

FASEB J

Rhythmic expression of miR-27b-3p targets the clock gene Bmal1 at the posttranscriptional level in the mouse liver.

Authors - Wenxiang Zhang
mouseWB

Commun Biol

ISX-9 potentiates CaMKIIδ-mediated BMAL1 activation to enhance circadian amplitude.

Authors - Huilin Li
rat,humanWB,IHC

Sci Rep

Gene expression profiles during tissue remodeling following bladder outlet obstruction.

Authors - Saya Ito
  • KD Validated
chickenIHC

Animals (Basel)

Renal Endocytic Regulation of Vitamin D Metabolism during Maturation and Aging in Laying Hens

Authors - Nami Kuwata