DCAF16 Polyclonal antibody

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  WB analysis using 32592-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32592-1-AP (DCAF16 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

DCAF16 Polyclonal Antibody for WB, ELISA

Cat No. 32592-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DDB1- and CUL4-associated factor 16, C4orf30

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Tested Applications

• Positive WB detected in: HCT 116 cells, HEK-293T cells, HuH-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32592-1-AP targets DCAF16 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag36770

  Product name: Recombinant human C4orf30 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC101716

  Sequence: MGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVPNLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSHCVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRATPIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL

• Full Name: chromosome 4 open reading frame 30
• Calculated Molecular Weight: 216 aa, 24 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC101716
• Gene Symbol: C4orf30
• Gene ID (NCBI): 54876
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9NXF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCAF16 is a member of the DDB1-CUL4 associated factor (DCAF) family. It is a vital component in the CUL4-DDB1 E3 ubiquitin-protein ligase complex, serving as a crucial substrate recognition element. DCAF16 plays a central role in the ubiquitination and subsequent proteasome-dependent degradation of nuclear proteins.