DCDC2 Polyclonal antibody

DCDC2 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 26978-1-AP

Synonyms

DCDC2, DCDC2A, KIAA1154, Protein RU2S, RU2, RU2S

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Validation Data Gallery

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  WB analysis of mouse placenta using 26978-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 26978-1-AP (DCDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 26978-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26978-1-AP (DCDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 26978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26978-1-AP (DCDC2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 26978-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26978-1-AP (DCDC2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 26978-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 26978-1-AP (DCDC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse placenta tissue, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 26978-1-AP is DCDC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCDC2 fusion protein Ag25577
• Full Name: doublecortin domain containing 2
• Calculated molecular weight: 476 aa, 53 kDa
• Observed molecular weight: 52 kDa
• GenBank accession number: BC050704
• Gene symbol: DCDC2
• Gene ID (NCBI): 51473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.