DCK Polyclonal antibody

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DCK Polyclonal Antibody for WB, ELISA

Cat No. 17758-1-AP Copied!

Publications (14)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Deoxyadenosine kinase, deoxycytidine kinase, Deoxyguanosine kinase, EC:2.7.1.113, EC:2.7.1.74

• 

  WB analysis using 17758-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17758-1-AP

  WB result of DCK antibody (17758-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DCK transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 17758-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 17758-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 17758-1-AP (DCK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, MCF-7 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17758-1-AP targets DCK in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12123

  Product name: Recombinant human DCK protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-260 aa of BC103764

  Sequence: MATPPKRSCPSFSASSEGTRIKKISIEGNIAAGKSTFVNILKQLCEDWEVVPEPVARWCNVQSTQDEFEELTMSQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESECMNETEWTIYQDWHDWMNNQFGQSLELDGIIYLQATPETCLHRIYLRGRNEEQGIPLEYLEKLHYKHESWLLHRTLKTNFDYLQEVPILTLDVNEDFKDKYESLVEKVKEFLSTL

• Full Name: deoxycytidine kinase
• Calculated Molecular Weight: 260 aa, 31 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC103764
• Gene Symbol: DCK
• Gene ID (NCBI): 1633
• RRID: AB_2261476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P27707
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Deoxycytidine kinase (dCK) is required for the phosphorylation of several deoxyribonucleoside analogues that are widely employed as chemotherapeutic agents(PMID:12628850). It is also a key enzyme in the phosphorylation of a variety of antineoplastic and antiviral nucleoside analogs including cytosine arabinoside (araC) and dideoxycytidine (ddCyd); deficiency of deoxycytidine kinase activity mediates resistance to these drugs(PMID:1996353). This protein can exsit as a homodimer(PMID:16421443).

Publications

What published studies show

DCK Polyclonal antibody (Cat# 17758-1-AP) has 14 citations across journals including Small, Oncogene, Materials Today Bio, Biochemical Pharmacology, Scientific Reports, International Journal of Cancer, ACS Pharmacology & Translational Science, Frontiers in Oncology, Investigative New Drugs, American Journal of Cancer Research, Leukemia Research, Journal of Gastrointestinal Oncology, and Aging. Research fields span pancreatic cancer ferroptosis, gemcitabine/cytarabine resistance mechanisms, pyrimidine metabolism, acute myeloid leukemia, and hepatocellular carcinoma prognosis.

Automatically generated from the 14 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Small; Nanomagnetic Hyperthermia Sensitizes Gemcitabine Chemosensitivity in Pancreatic Cancer by Inhibiting HSPB1 to Amplify ACSL4-Mediated Ferroptosis.; Authors - Jiaqiang Ren
human	WB	Mater Today Bio; Targeting FN1 to overcome gemcitabine resistance in gallbladder cancer: Mechanistic insights and an iRGD-modified PEG-PLGA nanoparticle delivery strategy.; Authors - Maojin Tian
human	WB	Oncogene; KNSTRN promotes tumorigenesis and gemcitabine resistance by activating AKT in bladder cancer.; Authors - Yaoyi Xiong
human	WB	Biochem Pharmacol; Loss of cystathionine-β-synthase contributes to elevated OXPHOS, a vulnerability in Ara-C-resistant Myeloid Leukemia in Down syndrome.; Authors - Jenna Thibodeau
		Biochem Pharmacol; Exploiting CDA-Mediated metabolism of 5fdC to overcome cytarabine resistance in Malignant myeloid cells leukemia.; Authors - Anran Fan
human	WB	Int J Cancer; Adult T-cell leukemia-lymphoma acquires resistance to DNA demethylating agents through dysregulation of enzymes involved in pyrimidine metabolism.; Authors - Nao Yoshida-Sakai