Decorin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83753-4-RR (same clone as 83753-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83753-4-RR (Decorin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 83753-4-RR (same clone as 83753-4-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 83753-4-RR (same clone as 83753-4-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug Decorin Recombinant antibody (83753-4-RR, Clone:240781E3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83753-4-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83753-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83753-4-RR against Human Decorin were performed. The affinity constant is below 1 pM.

Decorin Uni-rAb^® Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 83753-4-PBS

Clone No.240781E3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Synonyms

DCN, 240781E3, CSCD, DSPG2, PG S2

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Product Information

83753-4-PBS targets Decorin in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6275

  Product name: Recombinant human DCN protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-359 aa of BC005322

  Sequence: MKATIILLLLAQVSWAGPFQQRGLFDFMLEDEASGIGPEVPDDRDFEPSLGPVCPFRCQCHLRVVQCSDLGLDKVPKDLPPDTTLLDLQNNKITEIKDGDFKNLKNLHALILVNNKISKVSPGAFTPLVKLERLYLSKNQLKELPEKMPKTLQELRAHENEITKVRKVTFNGLNQMIVIELGTNPLKSSGIENGAFQGMKKLSYIRIADTNITSIPQGLPPSLTELHLDGNKISRVDAASLKGLNNLAKLGLSFNSISAVDNGSLANTPHLRELHLDNNKLTRVPGGLAEHKYIQVVYLHNNNISVVGSSDFCPPGHNTKKASYSGVSLFSNPVQYWEIQPSTFRCVYVRSAIQLGNYK

• Full Name: decorin
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC005322
• Gene Symbol: Decorin
• Gene ID (NCBI): 1634
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P07585
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. The binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. Decorin(~ 40 kDa) also binds glycosaminoglycan chains to form big molecules.