DCT Polyclonal antibody

DCT Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 13095-1-AP

Synonyms

DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2



Tested Applications

Positive WB detected inHL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13095-1-AP targets DCT in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCT fusion protein Ag3714
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated molecular weight 519 aa, 59 kDa
Observed molecular weight 59 kDa
GenBank accession numberBC028311
Gene symbol DCT
Gene ID (NCBI) 1638
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).

Protocols

Product Specific Protocols
WB protocol for DCT antibody 13095-1-APDownload protocol
IHC protocol for DCT antibody 13095-1-APDownload protocol
IP protocol for DCT antibody 13095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Gene Ther

Immunotargeting and eradication of orthotopic melanoma using a chemokine-enhanced DNA vaccine.

Authors - O Igoucheva
mouseWB

Int J Mol Sci

Inhibition of Melanogenesis by Gallic Acid: Possible Involvement of the PI3K/Akt, MEK/ERK and Wnt/β-Catenin Signaling Pathways in B16F10 Cells.

Authors - Su Tzu-Rong TR
mouseWB

Asian J Pharm Sci

A biomimetic antitumor nanovaccine based on biocompatible calcium pyrophosphate and tumor cell membrane antigens.

Authors - Minghui Li
humanWB

Cancer Lett

MiR-101 inhibits melanoma cell invasion and proliferation by targeting MITF and EZH2.

Authors - Luo Chonglin C
humanWB

Sci Rep

Loss of tumorigenic potential upon transdifferentiation from keratinocytic into melanocytic lineage.

Authors - Sabrina Fehrenbach
humanWB

J Invest Dermatol

miR-137 inhibits the invasion of melanoma cells through downregulation of multiple oncogenic target genes.

Authors - Chonglin Luo