DCT Polyclonal antibody

DCT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, monkey





Cat no : 13095-1-AP


DCT, DT, L dopachrome Delta isomerase, L dopachrome tautomerase, TRP 2, TRP2, Tyrosinase related protein 2, TYRP2

Tested Applications

Positive WB detected inHL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue
Positive IP detected inHL-60 cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13095-1-AP targets DCT in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Reactivity human, mouse, rat, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCT fusion protein Ag3714
Full Name dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)
Calculated Molecular Weight 519 aa, 59 kDa
Observed Molecular Weight 59 kDa
GenBank Accession NumberBC028311
Gene Symbol DCT
Gene ID (NCBI) 1638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).


Product Specific Protocols
WB protocol for DCT antibody 13095-1-APDownload protocol
IHC protocol for DCT antibody 13095-1-APDownload protocol
IF protocol for DCT antibody 13095-1-APDownload protocol
IP protocol for DCT antibody 13095-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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