|Positive WB detected in||HL-60 cells, A375 cells, A431 cells, COS-7 cells, MCF-7 cells, rat skin tissue|
|Positive IP detected in||HL-60 cells|
|Positive IHC detected in||human skin tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
13095-1-AP targets DCT in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DCT fusion protein Ag3714|
|Full Name||dopachrome tautomerase (dopachrome delta-isomerase, tyrosine-related protein 2)|
|Calculated molecular weight||519 aa, 59 kDa|
|Observed molecular weight||59 kDa|
|GenBank accession number||BC028311|
|Gene ID (NCBI)||1638|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DCT(L-dopachrome Delta-isomerase) is also named as TYRP2,TRP2 and belongs to the tyrosinase family.human TYRP2 protein has 83% identity and 90% similarity to the mouse sequence and has all the structural characteristics of the tyrosinase protein family, including a signal peptide, 15 conserved cysteine residues, 2 copper-binding domains, and a C-terminal membrane-spanning region(PMID:8206391).
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