DCTN4 Polyclonal antibody

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• DCTN4 Polyclonal antibody (17098-1-AP)

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• KD/KO Validated

DCTN4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17098-1-AP Copied!

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Dynactin subunit p62, Dynactin subunit 4, Dyn4

• 

  WB analysis of human brain using 17098-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 17098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 17098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Jurkat using 17098-1-AP

  IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 17098-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17098-1-AP (DCTN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17098-1-AP targets DCTN4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10579

  Product name: Recombinant human DCTN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 91-460 aa of BC026323

  Sequence: STQLTDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPHTQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSDKYGLGTRLQRPRAGASISTLAGLSLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKRSLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFECEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEGEVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP

• Full Name: dynactin 4 (p62)
• Calculated Molecular Weight: 460 aa, 52 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC026323
• Gene Symbol: DCTN4
• Gene ID (NCBI): 51164
• RRID: AB_2276944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJW0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The DCTN4 Polyclonal antibody (Cat# 17098-1-AP) has 2 citations. These appear in Molecular Biology of the Cell (2012) and Oncology Targets and Therapy (2020). The research fields span cell biology—specifically dynactin complex cargo targeting—and oncology, focusing on glioblastoma growth and metastasis via lncRNA/miRNA regulation. Both studies utilized the antibody for Western blot analysis.

Automatically generated from the 2 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Monkey	WB	Mol Biol Cell; Dynactin's pointed-end complex is a cargo-targeting module.; Authors - Ting-Yu Yeh ; KD Validated
human	WB	Onco Targets Ther; Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.; Authors - Xin Xing Li