DCTN4 Polyclonal antibody

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  WB analysis of human brain using 17098-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17098-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17098-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 17098-1-AP (DCTN4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 17098-1-AP

  IP result of anti-DCTN4 (IP:17098-1-AP, 4ug; Detection:17098-1-AP 1:700) with Jurkat cells lysate 2800ug.

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  IHC staining of human stomach using 17098-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 17098-1-AP (DCTN4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

DCTN4 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 17098-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, ELISA

Synonyms

Dynactin subunit p62, Dynactin subunit 4, Dyn4

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Tested Applications

• Positive WB detected in: human brain tissue, HepG2 cells, Jurkat cells
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

17098-1-AP targets DCTN4 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10579

  Product name: Recombinant human DCTN4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 91-460 aa of BC026323

  Sequence: STQLTDDPAKTTMKKAYYLACGFCRWTSRDVGMADKSVASGGWQEPENPHTQRMNKLIEYYQQLAQKEKVERDRKKLARRRNYMPLAFSDKYGLGTRLQRPRAGASISTLAGLSLKEGEDQKEIKIEPAQAVDEVEPLPEDYYTRPVNLTEVTTLQQRLLQPDFQPVCASQLYPRHKHLLIKRSLRCRKCEHNLSKPEFNPTSIKFKIQLVAVNYIPEVRIMSIPNLRYMKESQVLLTLTNPVENLTHVTLFECEEGDPDDINSTAKVVVPPKELVLAGKDAAAEYDELAEPQDFQDDPDIIAFRKANKVGIFIKVTPQREEGEVTVCFKMKHDFKNLAAPIRPIEESDQGTEVIWLTQHVELSLGPLLP

• Full Name: dynactin 4 (p62)
• Calculated Molecular Weight: 460 aa, 52 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC026323
• Gene Symbol: DCTN4
• Gene ID (NCBI): 51164
• RRID: AB_2276944
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJW0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
Monkey	WB	Mol Biol Cell; Dynactin's pointed-end complex is a cargo-targeting module.; Authors - Ting-Yu Yeh ; KD Validated
human	WB	Onco Targets Ther; Linc01094 Accelerates the Growth and Metastatic-Related Traits of Glioblastoma by Sponging miR-126-5p.; Authors - Xin Xing Li