Tested Applications
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10182-1-AP targets DCTN5 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0234 Product name: Recombinant human DCTN5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-182 aa of BC004191 Sequence: ELGELLYNKSEYIETASGNKVSRQSVLCGSQNIVLNGKTIVMNDCIIRGDLANVRVGRHCVVKSRSVIRPPFKKFSKGVAFFPLHIGDHVFIEEDCVVNAAQIGSYVHVGKNCVIGRRCVLKDCCKILDNTVLPPETVVPPFTVFSGCPGLFSGELPECTQELMIDVTKSYYQKFLPLTQV Predict reactive species |
| Full Name | dynactin 5 (p25) |
| Calculated Molecular Weight | 20 kDa |
| GenBank Accession Number | BC004191 |
| Gene Symbol | DCTN5 |
| Gene ID (NCBI) | 84516 |
| RRID | AB_2088344 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BTE1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN5 antibody 10182-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-Dynactin 1 (p50) antibody (Cat# 10182-1-AP) has 6 citations. These appear in Journal of Hazardous Materials, EMBO Journal, Current Biology, Scientific Reports, and Molecular Biology Cell (two papers). The associated research fields span cell biology and mitosis—particularly dynactin function, kinetochore targeting, and spindle orientation—as well as autophagy defects in renal cell carcinoma and the neurotoxic effects of nano-graphene oxide on mitochondrial transport.
| Species | Application | Title |
|---|---|---|
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit. | ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Sci Rep Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions. | ||
Reviews
What customers say
The single review rates this antibody 5 stars. It was used for Western Blot and Immunohistochemistry on human post-mortem tissue to detect DCTN5 protein expression in Alzheimer's disease and non-AD patient samples. The reviewer reported a clear, specific band around 20 kDa, consistent with the expected molecular weight of DCTN5, and expressed satisfaction with the antibody's reliability as a reagent.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (11-05-2025) | I use the DCTN5 antibody for detecting protein expression in Alzheimer's disease(AD) and non AD patient samples. The antibody showed a clear and specific band around 20kDa,consistent with the expected molecular weight of DCTN5.I am satisfied with the antibody, its reliable reagents.
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