DCTN5 Polyclonal antibody

• Featured Product
• KD/KO Validated

DCTN5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 10182-1-AP

Synonyms

DCTN5, dynactin 5 (p25), Dynactin subunit 5, Dynactin subunit p25, p25

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Validation Data Gallery

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  IHC staining of human breast cancer using 10182-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10182-1-AP (DCTN5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10182-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10182-1-AP (DCTN5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10182-1-AP targets DCTN5 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, rat, Monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCTN5 fusion protein Ag0234
• Full Name: dynactin 5 (p25)
• Calculated Molecular Weight: 20 kDa
• GenBank Accession Number: BC004191
• Gene Symbol: DCTN5
• Gene ID (NCBI): 84516
• RRID: AB_2088344
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
Monkey	WB	EMBO J; Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.; Authors - Yeh Ting-Yu TY
rat	WB	J Hazard Mater; Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.; Authors - Yiyuan Kang
human	WB	Curr Biol; An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation.; Authors - Florencia di Pietro
Monkey	WB	Mol Biol Cell; Dynactin's pointed-end complex is a cargo-targeting module.; Authors - Ting-Yu Yeh ; KD Validated
Monkey	WB	Mol Biol Cell; Dynactin integrity depends upon direct binding of dynamitin to Arp1.; Authors - Frances Ka Yan Cheong
human	IHC,IF	Sci Rep; Autophagy defects and related genetic variations in renal cell carcinoma with eosinophilic cytoplasmic inclusions.; Authors - Zhou Yu