Tested Applications
| Positive WB detected in | A549 cells, mouse lung tissue, mouse testis tissue, rat testis tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | mouse testis tissue, human kidney tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:600-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16947-1-AP targets DCTN6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10561 Product name: Recombinant human DCTN6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-190 aa of BC013175 Sequence: MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN Predict reactive species |
| Full Name | dynactin 6 |
| Calculated Molecular Weight | 190 aa, 21 kDa |
| Observed Molecular Weight | 27 kDa |
| GenBank Accession Number | BC013175 |
| Gene Symbol | DCTN6 |
| Gene ID (NCBI) | 10671 |
| RRID | AB_2245654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00399 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| IP protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| WB protocol for DCTN6 antibody 16947-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The antibody is the Dynactin 6 (p27) antibody, catalog number 16947-1-AP, with a total of 5 citations. These appear in EMBO Journal, Current Biology, Cell Reports, Neurobiology of Aging, and Molecular Biology of the Cell. The associated research fields span cell biology and mitosis (kinetochore targeting, spindle orientation), host-pathogen interactions (Chlamydia infection), neurobiology and aging (dystrophic neurite formation), and intracellular transport (retrograde axonal vesicle transport). Applications were primarily Western blot, with some IF and IP.
| Species | Application | Title |
|---|---|---|
EMBO J Dynactin helps target Polo-like kinase 1 to kinetochores via its left-handed beta-helical p27 subunit.
| ||
Curr Biol An RNAi Screen in a Novel Model of Oriented Divisions Identifies the Actin-Capping Protein Z β as an Essential Regulator of Spindle Orientation. | ||
Cell Rep The Chlamydia effector Dre1 binds dynactin to reposition host organelles during infection | ||
Neurobiol Aging Dynactin 6 deficiency enhances aging-associated dystrophic neurite formation in mouse brains.
| ||
Mol Biol Cell Mycalolide B dissociates dynactin and abolishes retrograde axonal transport of dense-core vesicles. |
Reviews
What customers say
The single review for this DCTN6 antibody (Rb/IgG) reports a 4-star rating. The user tested it for Western Blot and protein purification at a 1:1000 dilution on human post mortem tissue. They observed a clear reduction in DCTN6 expression, along with an interesting decrease in DCTN5 levels. Overall, the antibody was described as reliable and consistent in its performance, working as expected.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (09-23-2025) | The DCTN6 (Rb/IgG) worked as expected. we observed a clear reduction in dctn6 expression, and interestingly, a decrease in DCTN5 levels was also noted. Overall ,the antibody performed reliable and produced consistent results.
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