DCUN1D3 Polyclonal antibody

DCUN1D3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26356-1-AP

Synonyms

44M2.4, DCN1 like protein 3, DCUN1D3

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 26356-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 26356-1-AP (DCUN1D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 26356-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 26356-1-AP (DCUN1D3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: MCF-7 cells, A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26356-1-AP is DCUN1D3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DCUN1D3 fusion protein Ag23730
• Full Name: DCN1, defective in cullin neddylation 1, domain containing 3 (S. cerevisiae)
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC040442
• Gene Symbol: DCUN1D3
• Gene ID (NCBI): 123879
• RRID: AB_2880489
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DCUN1D3, also named as SCCRO3, is proposed to function as a tumor suppressor. DCUN1D3 antagonizes DCUN1D1-mediated CUL1 neddylation by sequestering CUL1 at the cell membrane (PubMed:25349211). When overexpressed in transformed cells, DCUN1D3 may promote mesenchymal to epithelial-like changes and inhibit colony formation in soft agar (PubMed:25349211).