|Positive WB detected in||fetal human brain tissue|
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
13925-1-AP targets DCX in WB, IF,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||DCX fusion protein Ag4945|
|Calculated molecular weight||402 aa, 45 kDa|
|Observed molecular weight||40 kDa|
|GenBank accession number||BC027925|
|Gene ID (NCBI)||1641|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It’s also a common neuron marker since its expression is specific to neurons.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sampath (Verified Customer) (12-02-2019)
It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.
Sheed (Verified Customer) (01-26-2019)
Works well with Edu click reaction
Eleonora (Verified Customer) (12-11-2018)
The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).