Tested Applications
| Positive WB detected in | SH-SY5Y cells, fetal human brain tissue |
| Positive IHC detected in | human gliomas tissue, mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
| Positive IF-Fro detected in | mouse brain tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13925-1-AP targets DCX in WB, IHC, IF/ICC, IF-P, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, hamster |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4945 Product name: Recombinant human DCX protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC027925 Sequence: MELDFGHFDERDKTSRNMRGSRMNGLPSPTHSAHCSFYRTRTLQALSNEKKAKKVRFYRNGDRYFKGIVYAVSSDRFRSFDALLADLTRSLSDNINLPQGVRYIYTIDGSRKIGSMDELEEGESYVCSSDNFFKKVEYTKNVNPNWSVNVKTSANMKAPQSLASSNSAQARENKDFVRPKLVTIIRSGVKPRKAVRVLLNKKTAHSFEQVLTDITEAIKLETGVVKKLYTLDGKQVTCLHDFFGDDDVFIACGPEKFRYAQDDFSLDENECRVMKGNPSATAGPKASPTPQKTSAKSPGPMRRSKSPADSANGTSSSQLSTPKSKQSPISTPTSPGSLRKHKDLYLPLSLDDSDSLGDSM Predict reactive species |
| Full Name | doublecortin |
| Calculated Molecular Weight | 402 aa, 45 kDa |
| Observed Molecular Weight | 40 kDa |
| GenBank Accession Number | BC027925 |
| Gene Symbol | DCX |
| Gene ID (NCBI) | 1641 |
| RRID | AB_2088476 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43602 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It's also a common neuron marker since its expression is specific to neurons.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DCX antibody 13925-1-AP | Download protocol |
| IHC protocol for DCX antibody 13925-1-AP | Download protocol |
| WB protocol for DCX antibody 13925-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DCX Polyclonal antibody (Cat# 13925-1-AP) has 80 citations published in 56 journals, including Nature Communications, Cell Host & Microbe, Biomaterials, Neural Regeneration Research, Aging Cell, Neurotherapeutics, and Journal of Neuroinflammation. Associated research fields encompass hippocampal neurogenesis, Alzheimer's disease, depression, ischemic stroke, traumatic brain injury, Parkinson's disease, neuroinflammation, gut-brain axis, neural stem cell differentiation, cognitive impairment, epilepsy, and glioma. Primary applications are IF, WB, and IHC across mouse, rat, human, and hamster models.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe A Gpr35-tuned gut microbe-brain metabolic axis regulates depressive-like behavior | ||
Nat Commun ASO therapy rescues NOTCH2NLC GGC repeat expansion-induced genomic damage, 3D chromatin structural abnormalities, and senescence. | ||
Nat Commun Ten-eleven translocation 1 mediated-DNA hydroxymethylation is required for myelination and remyelination in the mouse brain. | ||
Nat Commun m6a methylation orchestrates IMP1 regulation of microtubules during human neuronal differentiation | ||
Nat Commun Correction of dysregulated lipid metabolism normalizes gene expression in oligodendrocytes and prolongs lifespan in female poly-GA C9orf72 mice | ||
Adv Sci (Weinh) Taurocholic Acid Is Associated With Disturbed Functional Connectivity in the Hippocampus of Patients With Depression. |
Reviews
What customers say
Users report strong performance for this DCX antibody across applications. One reviewer achieved a strong signal in brain tissue Western blots at 1:1000 and highly recommends it for DCX detection. Another found it effective for immunofluorescence at 1:500, particularly compatible with EdU click reactions in mouse tissue. A third user confirmed good WB results at 1:600 in mouse cortex and hippocampus but noted some non-specific interaction with a protein ladder. Overall, the antibody is well-regarded for sensitivity and reliability in both WB and IF.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Sampath (Verified Customer) (12-02-2019) | It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.
|
Sheed (Verified Customer) (01-26-2019) | Works well with Edu click reaction
|
Eleonora (Verified Customer) (12-11-2018) | The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).
 |
