DCX Polyclonal antibody

DCX Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 13925-1-AP

Synonyms

DBCN, DC, DCX, doublecortin, Doublin, Lis X, Lissencephalin X, LISX, SCLH, XLIS



Tested Applications

Positive WB detected infetal human brain tissue
Positive IF detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

13925-1-AP targets DCX in WB, IF,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DCX fusion protein Ag4945
Full Name doublecortin
Calculated molecular weight 402 aa, 45 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC027925
Gene symbol DCX
Gene ID (NCBI) 1641
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DCX (Doublecortin) is a microtubule-associated protein that is essential for normal brain development. DCX is expressed in migrating neurons throughout the central and peripheral nervous system during embryonic and postnatal development. Anti-DCX antibodies are widely used for detection of neurogenesis. It’s also a common neuron marker since its expression is specific to neurons.

Protocols

Product Specific Protocols
WB protocol for DCX antibody 13925-1-APDownload protocol
IF protocol for DCX antibody 13925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Cell Neurosci

HDAC1 Silence Promotes Neuroprotective Effects of Human Umbilical Cord-Derived Mesenchymal Stem Cells in a Mouse Model of Traumatic Brain Injury via PI3K/AKT Pathway.

Authors - Ling Xu
humanIF

Brain Res

G Protein-Coupled Estrogen Receptor 1 Negatively Regulates the Proliferation of Mouse-Derived Neural Stem/Progenitor Cells via Extracellular Signal-regulated Kinase Pathway.

Authors - Jun Zhong
mouseIF

Biochem Biophys Res Commun

Identification and immunohistochemical characterization of G-quadruplexes in mouse brain.

Authors - Sefan Asamitsu
ratWB

Biomed Pharmacother

Astragaloside IV improves neurobehavior and promotes hippocampal neurogenesis in MCAO rats though BDNF-TrkB signaling pathway.

Authors - Guang-Xiao Ni

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sampath (Verified Customer) (12-02-2019)

It worked very well brain samples and gave me a strong signal for DCX at 1:1000 dilution for western blots. I had to increase the dilution to 1:2000. This is the antibody I was looking for. If you are looking for DCX antibody, I will recommend this.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
FH

Sheed (Verified Customer) (01-26-2019)

Works well with Edu click reaction

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse
FH

Eleonora (Verified Customer) (12-11-2018)

The antibody works well, but it has too much interaction with the protein ladder (I have used Fisher BioReagent EZ-RUN).

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:600
  • Cell Tissue Type: cortex (right) and hippocampus (left) mouse 3 weeks
DCX Antibody Western Blot, validation (1:600 dilution) in cortex (right) and hippocampus (left) mouse 3 weeks (Cat no:13925-1-AP)