DCXR Polyclonal antibody

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• DCXR Polyclonal antibody (15188-1-AP)

DCXR Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15188-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Carbonyl reductase II, DCR, DCXR, HCR2, HCRII, KIDCR, Kidney dicarbonyl reductase, L xylulose reductase, P34H, SDR20C1, Sperm surface protein P34H, XR

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  WB analysis of human testis using 15188-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15188-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15188-1-AP (DCXR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human testis using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15188-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15188-1-AP (DCXR Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, human testis tissue, human liver tissue, mouse testis tissue
• Positive IHC detected in: human kidney tissue, human liver tissue, human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15188-1-AP targets DCXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7272

  Product name: Recombinant human DCXR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-244 aa of BC001470

  Sequence: MELFLAGRRVLVTGAGKGIGRGTVQALHATGARVVAVSRTQADLDSLVRECPGIEPVCVDLGDWEATERALGSVGPVDLLVNNAAVALLQPFLEVTKEAFDRSFEVNLRAVIQVSQIVARGLIARGVPGAIVNVSSQCSQRAVTNHSVYCSTKGALDMLTKVMALELGPHKIRVNAVNPTVVMTSMGQATWSDPHKAKTMLNRIPLGKFAEVEHVVNAILFLLSDRSGMTTGSTLPVEGGFWAC

• Full Name: dicarbonyl/L-xylulose reductase
• Calculated Molecular Weight: 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC001470
• Gene Symbol: DCXR
• Gene ID (NCBI): 51181
• RRID: AB_2088504
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z4W1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The DCXR Polyclonal antibody (Cat# 15188-1-AP) has 3 total citations. These appear in Molecular Psychiatry, International Journal of Surgery, and Environmental Research. The associated research fields include neuroscience (synaptic plasticity and antidepressant strategies), oncology (hepatocellular carcinoma prognosis and tumor microenvironment), and reproductive toxicology (arsenic exposure effects on spermatogenesis).

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IHC	Mol Psychiatry; Targeting HINT1 to improve synaptic plasticity: toward loganin as a new antidepressant strategy; Authors - Congyuan Xia
human	WB	Int J Surg; Decoding hepatocellular carcinoma prognosis: a machine learning-derived methylation signature integrating transcriptomic and tumor microenvironment insights.; Authors - Hong Song
mouse	WB	Environ Res; Prenatal and postnatal exposure to environmental level arsenic declines male reproductive function by affecting spermatogenesis and maturation in offspring.; Authors - Xiaochao Song