DDAH2 Monoclonal antibody

DDAH2 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Rat, Mouse, Rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1A10G3

Cat no : 68154-1-Ig

Synonyms

DDAH, DDAH 2, DDAH2, DDAHII, Dimethylargininase 2, G6a, NG30, Protein G6a, S phase protein

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Validation Data Gallery

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  WB analysis using 68154-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse retina using 68154-1-Ig

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat colon using 68154-1-Ig

  rat colon tissue were subjected to SDS PAGE followed by western blot with 68154-1-Ig (DDAH2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: rabbit brain tissue, mouse retina tissue, rat colon tissue, rat brain tissue, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68154-1-Ig targets DDAH2 in WB, ELISA applications and shows reactivity with Human, Rat, Mouse, Rabbit samples.

• Tested Reactivity: Human, Rat, Mouse, Rabbit
• Host / Isotype: Mouse / IgG3
• Class: Monoclonal
• Type: Antibody
• Immunogen: DDAH2 fusion protein Ag7127
• Full Name: dimethylarginine dimethylaminohydrolase 2
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001435
• Gene Symbol: DDAH2
• Gene ID (NCBI): 23564
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The DDAH family of proteins is formed by two isoforms, DDAH-1 and DDAH-2, both of which utilize ADMA as their substrate and increase NOS activity. DDAH-2,also named as DDAH, G6A, NG30, is the most prevalent isoform in blood vessels and endothelium and is important for embryonic development. It has also been linked to other diseases, such as hypertension, cerebral hemorrhage, cardiac disease, and diabetes. In cultured human endothelial cells,DDAH-2 had been found only in the cytosol, but in the nuclei of rat vascular smooth muscle cells.