DDB2 Polyclonal antibody

DDB2 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 10431-1-AP

Synonyms

DDB p48 subunit, DDB2, DDBB, DNA damage binding protein 2, UV DDB 2, UV DDB2



Tested Applications

Positive WB detected inHeLa cells, A431 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10431-1-AP targets DDB2 in WB, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDB2 fusion protein Ag0691
Full Name damage-specific DNA binding protein 2, 48kDa
Calculated molecular weight 48 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberBC000093
Gene symbol DDB2
Gene ID (NCBI) 1643
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

DDB2, also named as DNA damage-binding protein 2 or UV-damaged DNA-binding protein 2, is a 427 amino acid protein, which contains seven WD repeats and belongs to the WD repeat DDB2/WDR76 family. DDB2 is ubiquitously expressed, with highest levels in corneal endothelium and lowest levels in brain. DDB2 localizes in the nucleus and is required for DNA repair. DDB2 regulates negatively the constitutive expression of the SOD2 gene in breast cancer cells and exerts, at least in part, a control of breast cancer cell growth. DDB2 can play a role as oncogene and may become a promising candidate as a predictive marker in breast cancer.

Protocols

Product Specific Protocols
WB protocol for DDB2 antibody 10431-1-APDownload protocol
IF protocol for DDB2 antibody 10431-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cancer Genomics Proteomics

Surface plasmon resonance (SPR) spectrometry as a tool to analyze nucleic acid-protein interactions in crude cellular extracts.

Authors - Ahmed Farid E FE