DDI1 Polyclonal antibody

DDI1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 13968-1-AP

Synonyms

DDI1, Protein DDI1 homolog 1

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Validation Data Gallery

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  WB analysis using 13968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 13968-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 13968-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 13968-1-AP (DDI1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse testis tissue, U-937 cells, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

13968-1-AP targets DDI1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDI1 fusion protein Ag4992
• Full Name: DDI1, DNA-damage inducible 1, homolog 1 (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 47 kDa, 56 kDa
• GenBank Accession Number: BC022017
• Gene Symbol: DDI1
• Gene ID (NCBI): 414301
• RRID: AB_2089244
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ddi1(DNA-damage inducible 1)appears to release substrate from an ubiquitination complex, making the substrate available for deubiquitination to facilitate proteasomal degradation. Ddi1 could be a potential selective target for lung cancer treatment. Ddi1 runs as a doublet of 54/56 kDa on acrylamide gels besides the caculated MW 44 kDa.

Publications

Species	Application	Title
human	WB	J Mol Histol; Silibinin ameliorates cisplatin-induced acute kidney injury via activating Nfe2l1-mediated antioxidative response to suppress the ROS/MAPK signaling pathway.; Authors - Fang Yang
sheep	WB	Int J Mol Sci; The Effects of DDI1 on Inducing Differentiation in Ovine Preadipocytes via Oar-miR-432; Authors - Meilin Jin ; KO Validated