DDOST Polyclonal antibody

DDOST Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 14916-1-AP

Synonyms

AGE R1, DDOST, DDOST 48 kDa subunit, KIAA0115, OK/SW cl.45, OST, OST48, WBP1



Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inmouse testis tissue, rat small intestine tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

14916-1-AP targets DDOST in WB, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DDOST fusion protein Ag6727
Full Name dolichyl-diphosphooligosaccharide-protein glycosyltransferase
Calculated molecular weight 49 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC002594
Gene symbol DDOST
Gene ID (NCBI) 1650
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for DDOST antibody 14916-1-APDownload protocol
IHC protocol for DDOST antibody 14916-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Chem Biol

Oligosaccharyltransferase inhibition induces senescence in RTK-driven tumor cells.

Authors - Cecilia Lopez-Sambrooks