DDX10 Polyclonal antibody, PBS Only

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• DDX10 Polyclonal antibody, PBS Only (17857-1-PBS)

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  WB analysis of SMMC-7721 using 17857-1-AP (same clone as 17857-1-PBS)

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 17857-1-AP (same clone as 17857-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 17857-1-AP (DDX10 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17857-1-AP (DDX10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 17857-1-AP (same clone as 17857-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17857-1-AP (DDX10 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 17857-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

DDX10 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 17857-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

DEAD box protein 10, EC:3.6.4.13, HRH J8, Probable ATP-dependent RNA helicase DDX10

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Product Information

17857-1-PBS targets DDX10 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12317

  Product name: Recombinant human DDX10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-431 aa of BC091521

  Sequence: IGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLL

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Calculated Molecular Weight: 875 aa, 101 kDa
• Observed Molecular Weight: 101 kDa
• GenBank Accession Number: BC091521
• Gene Symbol: DDX10
• Gene ID (NCBI): 1662
• RRID: AB_2878454
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13206
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).