DDX10 Recombinant monoclonal antibody

• 

  WB analysis of SGC-7901 using 85709-1-RR

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 85709-1-RR (DDX10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

DDX10 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85709-1-RR

Clone No.242988B10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

DEAD box protein 10, EC:3.6.4.13, HRH J8, Probable ATP-dependent RNA helicase DDX10

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Tested Applications

• Positive WB detected in: SGC-7901 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85709-1-RR targets DDX10 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20766

  Product name: Recombinant human DDX10 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 100-431 aa of BC091521

  Sequence: IGLALQGKDVLGAAKTGSGKTLAFLVPVLEALYRLQWTSTDGLGVLIISPTRELAYQTFEVLRKVGKNHDFSAGLIIGGKDLKHEAERINNINILVCTPGRLLQHMDETVSFHATDLQMLVLDEADRILDMGFADTMNAVIENLPKKRQTLLFSATQTKSVKDLARLSLKNPEYVWVHEKAKYSTPATLEQNYIVCELQQKISVLYSFLRSHLKKKSIVFFSSCKEVQYLYRVFCRLRPGVSILALHGRQQQMRRMEVYNEFVRKRAAVLFATDIAARGLDFPAVNWVLQFDCPEDANTYIHRAGRTARYKEDGEALLILLPSEKAMVQQLL

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• Calculated Molecular Weight: 875 aa, 101 kDa
• Observed Molecular Weight: 101-110 kDa
• GenBank Accession Number: BC091521
• Gene Symbol: DDX10
• Gene ID (NCBI): 1662
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13206
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX10, DEAD-box helicase 10, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are involved in cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. Fusion of this gene and the nucleoporin gene NUP98 is found in leukemogenesis (PMID:20339440).