DDX11 Polyclonal antibody

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  WB analysis using 13047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13047-1-AP (DDX11 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

DDX11 Polyclonal Antibody for WB, ELISA

Cat No. 13047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

ATP-dependent DNA helicase DDX11, CHL1-related protein 1, DDX 11, DEAD/H-box protein 11, EC:3.6.4.12

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13047-1-AP targets DDX11 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3666

  Product name: Recombinant human DDX11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-345 aa of BC011264

  Sequence: MANETQKVGAIHFPFPFTPYSIQEDFMAELYRVLEAGKIGIFESPTGTGKSLSLICGALSWLRDFEQKKREEEARLLETGTGPLHDEKDESLCLSSSCEGAAGTPRPAGEPAWVTQFVQKKEERDLVDRLKAEQARRKQREERLQQLQHRVQLKYAAKRLRQEEEERENLLRLSREMLETGPEAERLEQLESGEEELVLAEYESDEEKKVASRVDEDEDDLEEEHITKIYYCSRTHSQLAQFVHEVKKSPFGKDVRLVSLGSRQNLCVNEDVKSLGSVQLINDRCVDMQRSRHEKKKGAEEEKPKRRRQEKQAACPFYNHEQMGLLRDEALAEVKDMEQLLALGK

• Full Name: DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S. cerevisiae)
• Calculated Molecular Weight: 970 aa, 108 kDa
• Observed Molecular Weight: 102-108 kDa
• GenBank Accession Number: BC011264
• Gene Symbol: DDX11
• Gene ID (NCBI): 1663
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96FC9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX11 (DEAD/H-box helicase 11), also known as hCHLR1, KRG-2, is a member of the DExD/H (Asp-Glu-x-Asp/His)-box helicase family containing DNA helicase with roles in DNA replication and sister chromatid cohesion establishment, and general chromosome architecture (PMID: 33802088). DDX11 encodes an iron-sulfur cluster DNA helicase required for development, mutated, and overexpressed in cancers (PMID: 33879618). DDX11 is also a positive regulator of the RIG-I-mitochondrial antiviral signaling protein (MAVS)-mediated signaling pathways (PMID: 39470258).