DDX18 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 85789-2-RR (same clone as 85789-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85789-2-RR (DDX18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02107-2

  Sandwich ELISA standard curve of MP02107-2, Human DDX18 Recombinant Matched Antibody Pair - PBS only. 85789-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag27457. 85789-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP02107-2

  The mean DDX18 concentration was determined to be 249.8 ng/mL in HEK-293T cell extract based on a 2.8 mg/mL extract load, 244.1 ng/mL in K-562 cell extract based on a 2.5 mg/mL extract load and 166.4 ng/mL in HepG2 cell extract based on a 1.8 mg/mL extract load.

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  IF Staining of HepG2 using 85789-2-RR (same clone as 85789-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85789-2-RR (same clone as 85789-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX18 antibody (85789-2-RR, Clone: 250053A4 ) at dilution of 1:2500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85789-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP02107-1

  Cytometric bead array standard curve of MP02107-1, DDX18 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85789-2-PBS. Detection antibody: 85789-1-PBS. Standard: Ag27457. Range: 1.563-200 ng/mL.

DDX18 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85789-2-PBS

Clone No.250053A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

ATP-dependent RNA helicase DDX18, cPERP-D, DEAD box protein 18, EC:3.6.4.13, MrDb

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Product Information

85789-2-PBS targets DDX18 as part of a matched antibody pair:

MP02107-1: 85789-2-PBS capture and 85789-1-PBS detection (validated in Cytometric bead array)

MP02107-2: 85789-2-PBS capture and 85789-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: DDX18 fusion protein Ag27457
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 18
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC001238
• Gene Symbol: DDX18
• Gene ID (NCBI): 8886
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NVP1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

DDX18 (DEAD-box helicase 18) is a protein belonging to the DEAD-box deconjugating enzyme family that is widely involved in RNA metabolism. It plays a key role in the assembly of the ribosomal small subunit (SSU) and ensures efficient intracellular protein synthesis. DDX18 unwinds RNA double strands through its deconjugating enzyme activity, which is essential for the proper processing and functional execution of RNA molecules. In addition, DDX18 is a multifunctional RNA deconjugating enzyme involved in a variety of signaling pathways, playing a key role not only in ribosome assembly and RNA metabolism, but also in the regulation of a variety of biological processes such as cell cycle, genome stability and stem cell pluripotency.