DDX27 Polyclonal antibody

DDX27 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Reviews (1)

Cat no : 17087-1-AP

Synonyms

Probable ATP-dependent RNA helicase DDX27, PP3241, HSPC259, EC:3.6.4.13, DEAD box protein 27

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Validation Data Gallery

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  WB analysis of HeLa using 17087-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 17087-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 17087-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 17087-1-AP (DDX27 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 17087-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 17087-1-AP (DDX27 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 17087-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using DDX27 antibody (17087-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse liver tissue, NIH/3T3 cells
• Positive IF/ICC detected in: HEK-293 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

17087-1-AP targets DDX27 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX27 fusion protein Ag9426
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 27
• Calculated Molecular Weight: 796 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC016060
• Gene Symbol: DDX27
• Gene ID (NCBI): 55661
• RRID: AB_2092569
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX27 is one of the DEAD box proteins, which is characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD). DEAD box proteins are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division, and DDX27 is involved in the processing of 5.8S and 28S ribosomal RNAs.

Publications

Species	Application	Title
human	WB	Mol Cell; Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity.; Authors - Francesca Rossi
human	WB	Onco Targets Ther; DEAD-box helicase 27 plays a tumor-promoter role by regulating the stem cell-like activity of human colorectal cancer cells.; Authors - Chunxing Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Wael (Verified Customer) (05-24-2023)

we detected the relative abundance of DDX27 in both the Nuclear and cytoplasmic fractions

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hek293

DDX27 Antibody Western Blot validation (1:1000 dilution) in Hek293 (Cat no:17087-1-AP)