Tested Applications
| Positive WB detected in | HEK-293T cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11617-1-AP targets DDX28 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2194 Product name: Recombinant human DDX28 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 191-540 aa of BC024273 Sequence: LGQPSLDSLPIPAPRGLVLVPSRELAQQVRAVAQPLGRSLGLLVRDLEGGHGMRRIRLQLSRQPSADVLVATPGALWKALKSRLISLEQLSFLVLDEADTLLDESFLELVDYILEKSHIAEGPADLEDPFNPKAQLVLVGATFPEGVGQLLNKVASPDAVTTITSSKLHCIMPHVKQTFLRLKGADKVAELVHILKHRDRAERTGPSGTVLVFCNSSSTVNWLGYILDDHKIQHLRLQGQMPALMRVGIFQSFQKSSRDILLCTDIASRGLDSTGVELVVNYDFPPTLQDYIHRAGRVGRVGSEVPGTVISFVTHPWDVSLVQKIELAARRRRSLPGLASSVKEPLPQAT Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 28 |
| Calculated Molecular Weight | 540 aa, 60 kDa |
| Observed Molecular Weight | 60 kDa |
| GenBank Accession Number | BC024273 |
| Gene Symbol | DDX28 |
| Gene ID (NCBI) | 55794 |
| RRID | AB_2092574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NUL7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DDX28 antibody 11617-1-AP | Download protocol |
| IHC protocol for DDX28 antibody 11617-1-AP | Download protocol |
| WB protocol for DDX28 antibody 11617-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DDX28 Polyclonal antibody (Cat# 11617-1-AP) has 2 citations. They appear in Cell Reports (2021), studying homeostatic control of nuclear-encoded mitochondrial gene expression and neuronal survival in mouse, and Translational Oncology (2026), investigating DDX28's role in cell cycle, DNA repair, and immunosuppressive microenvironment in acute myeloid leukemia in human. Both citations used the antibody for Western blot. The research fields span mitochondrial biology, neurobiology, oncology, and immunology.
| Species | Application | Title |
|---|---|---|
Cell Rep Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival. | ||
Transl Oncol Mitochondrial RNA helicase DDX28 promotes cell cycle and DNA repair programs and shapes an immunosuppressive microenvironment in acute myeloid leukemia.
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