DDX28 Polyclonal antibody

DDX28 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11617-1-AP

Synonyms

DDX28, MDDX28

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Validation Data Gallery

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  WB analysis using 11617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11617-1-AP (DDX28 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 11617-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11617-1-AP (DDX28 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: HEK-293T cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11617-1-AP targets DDX28 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX28 fusion protein Ag2194
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 28
• Calculated Molecular Weight: 540 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC024273
• Gene Symbol: DDX28
• Gene ID (NCBI): 55794
• RRID: AB_2092574
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Cell Rep; Homeostatic control of nuclear-encoded mitochondrial gene expression by the histone variant H2A.Z is essential for neuronal survival.; Authors - Christopher Lowden