DDX39A Polyclonal antibody

DDX39A Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 11723-1-AP

Synonyms

BAT1, BAT1L, DDX39, DDX39A, DDXL, DEAD box protein 39, Nuclear RNA helicase URH49, URH49

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Validation Data Gallery

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  WB analysis using 11723-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11723-1-AP (DDX39A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11723-1-AP

  IP result of anti-DDX39A (IP:11723-1-AP, 4ug; Detection:11723-1-AP 1:1000) with HEK-293 cells lysate 3000ug.

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  IHC staining of mouse kidney using 11723-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11723-1-AP (DDX39A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11723-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using DDX39A antibody (11723-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

11723-1-AP targets DDX39A in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DDX39A fusion protein Ag2311
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 39
• Calculated Molecular Weight: 427 aa, 49 kDa
• Observed Molecular Weight: 49-55 kDa
• GenBank Accession Number: BC032128
• Gene Symbol: DDX39
• Gene ID (NCBI): 10212
• RRID: AB_10858318
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX39A, also named the BAT1 protein, contains the nine conserved motifs that characterize the DEAD-box family of RNA-binding proteins. The family includes proteins found in all eukaryotic cell types, with considerable divergence in the sequences lying between the conserved motifs. Some of the motifs were known before the definition of the family and are responsible for binding to mRNA or ATP, or possess ATPase activity. Phylogenetic analyses have grouped BAT1 with the defining member of the DEAD-box family, eIF-4. This is a translation initiation factor required for the dissociation of stem/loop structures in mRNA at the ribosomes. This antibody may cross-react with DDX39B.

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res; A novel all-in-one conditional knockout system uncovered an essential role of DDX1 in ribosomal RNA processing.; Authors - Teruhiko Suzuki
human	WB,IHC	Int J Biol Sci; DDX39 as a predictor of clinical prognosis and immune checkpoint therapy efficacy in patients with clear cell renal cell carcinoma.; Authors - Yewei Bao
human	WB	Cell Rep; Proteomics reveal cap-dependent translation inhibitors remodel the translation machinery and translatome.; Authors - J J David Ho
human,chicken	IF,WB	PLoS Pathog; TREX (transcription/export)-NP complex exerts a dual effect on regulating polymerase activity and replication of influenza A virus; Authors - Lingcai Zhao ; KD Validated
human	IP,WB,IF	Mol Cell; 1The RNA helicase DDX39A binds a conserved structure in chikungunya virus RNA to control infection; Authors - Iulia Tapescu ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Robin (Verified Customer) (12-12-2019)

Great signal but specificity is questionable in comparison to UAP56 (ddx39B)

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: mouse fibroblasts