DDX41 Polyclonal antibody

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• DDX41 Polyclonal antibody (27500-1-AP)

DDX41 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27500-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

ABS, DEAD box protein 41, EC:3.6.4.13, Probable ATP-dependent RNA helicase DDX41

• 

  WB analysis using 27500-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 27500-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of RAW 264.7 using 27500-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 27500-1-AP (DDX41 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary cancer using 27500-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27500-1-AP (DDX41 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, RAW 264.7 cells, HepG2 cells, mouse ovary tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27500-1-AP targets DDX41 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag26593

  Product name: Recombinant human DDX41 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-144 aa of BC015476

  Sequence: MEESEPERKRARTDEVPAGGSRSEAEDEDDEDYVPYVPLRQRRQLLLQKLLQRRRKGAAEEEQQDSGSEPRGDEDDIPLGPQSNVSLLDQHQHLKEKAEARKESAKEKQLKEEEKILESVAEGRALMSVKEMAKGITYDDPIKT

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 41
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC015476
• Gene Symbol: DDX41
• Gene ID (NCBI): 51428
• RRID: AB_2880891
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UJV9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX41, also named as DEAD box protein abstrakt homolog, is a 622 amino acid protein, which is a probable ATP-dependent RNA helicase. DDX41 is required during post-transcriptional gene expression.and may be involved in pre-mRNA splicing.

Publications

What published studies show

DDX41 antibody (Cat# 27500-1-AP) has 3 total citations. These appear in NPJ Precision Oncology, Journal of Translational Medicine, and British Journal of Cancer. The research fields span head and neck squamous cell carcinoma (immune escape via STING pathway), Parkinson's disease (microglia-mediated neuroinflammation), and ovarian cancer (malignant progression through intron retention). Applications include Western blot and immunohistochemistry in human and mouse samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IHC	NPJ Precis Oncol; DDX41 facilitates PD-L1-mediated immune escape in OSCC via the phase separation and activation STING pathway.; Authors - Zhen Tian ; KD Validated
mouse	WB	J Transl Med; MicroRNA-218-5p-Ddx41 axis restrains microglia-mediated neuroinflammation through downregulating type I interferon response in a mouse model of Parkinson's disease; Authors - Danlei Wang
		Br J Cancer; Spliceosomal GTPase EFTUD2 mediates DDX41 intron retention to promote the malignant progression of ovarian cancer; Authors - Yanling Liu