DDX42 Polyclonal antibody

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  WB analysis using 31211-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31211-1-AP (DDX42 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 31211-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 31211-1-AP (DDX42 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 31211-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using DDX42 antibody (31211-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

DDX42 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31211-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RNA helicase related protein, RNA helicase like protein, RHELP, EC:3.6.4.13, DEAD box protein 42

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Tested Applications

• Positive WB detected in: HeLa cells, Jurkat cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31211-1-AP targets DDX42 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag34762

  Product name: Recombinant human DDX42 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 456-819 aa of BC015505

  Sequence: VARDIDTHTHRIGRTGRAGEKGVAYTLLTPKDSNFAGDLVRNLEGANQHVSKELLDLAMQNAWFRKSRFKGGKGKKLNIGGGGLGYRERPGLGSENMDRGNNNVMSNYEAYKPSTGAMGDRLTAMKAAFQSQYKSHFVAASLSNQKAGSSAAGASGWTSAGSLNSVPTNSAQQGHNSPDSPVTSAAKGIPGFGNTGNISGAPVTYPSAGAQGVNNTASGNNSREGTGGSNGKRERYTENRGSSRHSHGETGNRHSDSPRHGDGGRHGDGYRHPESSSRHTDGHRHGENRHGGSAGRHGENRGANDGRNGESRKEAFNRESKMEPKMEPKVDSSKMDKVDSKTDKTADGFAVPEPPKRKKSRWDS

• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 42
• Calculated Molecular Weight: 103 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC015505
• Gene Symbol: DDX42
• Gene ID (NCBI): 11325
• RRID: AB_3669903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q86XP3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DDX42 (DEAD (Asp-Glu-Ala-Asp) box polypeptide 42) is a member of the DEAD box RNA helicase superfamily. A C-terminal portion of DDX42 interacts with the pro-apoptotic factor, apoptosis-stimulating protein of p53 protein 2 (ASPP2), thereby inhibiting its action (PMID: 30834153).